| 摘要: |
| 用AIVH5N1和H9N2毒株作抗原，采取分步乳化法制成双乳相灭活疫苗。经无菌检验、稳定性和安全性试验，疫苗A和C符合兽用生物药品规程的要求，经免疫效力和保护力测定，疫苗可于免疫后3～5 d产生HI抗体，9～14 d抗体达高峰值，其中H5N1的HI抗体可达9 log2以上，H9N2可达11 log2以上，疫苗保护力与常规疫苗相近，但产生保护力的时间明显提早；疫苗在4 ℃条件下，保存期可达1年，免疫期为6～9个月。 |
| 关键词: 禽流感 双相疫苗 灭活疫苗 |
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| 基金项目:陕西省科技厅科技攻关项目(99K06-G3)；西北农林科技大学青年科研专项基金资助项目 |
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| Study on inactivated biphasic lacteal vaccines against avian influenza |
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| Abstract: |
| In this research inactivated biphasic lacteal vaccines was prepared using antigen with avian influenza virus (AIV)H5N1 and H9N2 subtypes.According to the test of sterility,stability and safety,A and C vaccine measured up to the demands of the rule of animal biological medicine.The result of the test of immunity and protection effect shows,its antibody (HI) could be produced 3-5 days after immunization,and reached its peaks within 9-14 days,The antibody (HI) of H5N1 could be reached 9 log2,and H9N2 could be reached 11 log2.Its protection effect was equal to the conventionl vaccine,but the time period of protection was advanced.At 4 ℃,it could be stocked for one year,and the immune period could last for 6-9 months. |
| Key words: avian influenza biphasic lacteal vaccine inactivated vaccine |
