| 摘要: |
| 将妊娠17 d山羊子宫内膜淋巴细胞（EML）放入含不同剂量PHA－P，CD58和雌酮的介质中进行体外培养，并分别用生物学方法和双抗体夹心ELISA法测定培养上清液中IL－2和IL－4的水平。结果表明，CD58和柴酮处理组EML分布的IL－2水平低，与细胞对照组差异不显著，并显著低于PHA－P处理组。3个浓度CD58处理组IL－4分泌水平高达5.40～6.36 ng/mL，高浓度雌酮处理组IL－4分泌量也达到4.77 ng/mL，与细胞对照组差异均极显著，而且CD58能以剂量依赖方式促进EML对IL－4的分泌。 |
| 关键词: 子宫内膜淋巴细胞（EML） IL-2 IL-4 CD58 雌酮 生殖调控 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39770545) |
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| Effects of CD58 and estrone on the secretion of IL-2 and IL-4 by goat EML in early gestation |
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| Abstract: |
| Endometrial lymphocytes (EMLs) of pregnant goat (17d) were cultured in vitro respectively in media containing PHA-P,CD58 and estrone at different doses.Then IL-2 and IL-4 in medium supernatants were measured respectively with biological method and double-antibody ELISA.The results showed that EMLs treated with CD58 and estrone secreted IL-2 at low level are insignificantly different from that of control cell group and significantly lower than that of EMLs treated with PHA-P.The EMLs treated with CD58 in three concentrations dose-dependently produced 5.40-6.36 ng/mL of IL-4 and those with estrone at higher dose produced 4.77 ng/mL of IL-4,all significantly higher than that of control cell group. |
| Key words: EML IL-2 IL-4 CD58 estrone reproductive control |
