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半胱胺对鹅胰液分泌及其脂肪酶活性的影响
艾晓杰1, 韩正康2
1.上海交通大学 农业与生物学院动物科学系;2.南京农业大学 农业部动物生理生化重点开放实验室
摘要:
12只装有胰腺-十二指肠长久性瘘管的成年鹅,圈养,夜间(20:00~8:00)禁食,随意饮水,试验采用前后自身对照,试验期在日粮中每千克体重一次性添加半胱胺100 mg,连续计量3 d对照期和8 d试验期的胰液分泌速度并测定其中胰脂肪酶活性,结果表明,添加半胱胺后,(1)胰液连续7 d的分泌速率较对照期极显著上升,幅度为71.26%~399.77%,其中白天升高了40.80%~456.09%,夜间增加了143.74%~311.42%,(2)单位容积胰肪酶的平均活性较对照均有不同程度的下降,变化幅度具有昼夜的差别,白天在第3~5天和第7天有升高的趋势(P>0.05),而夜间则显著低于对照,(3)每小时胰液中的脂肪酶总活性在第1~5天显著高于对照(P<0.01),且白天增加的幅度较夜晚大,说明半胱胺 能促进鹅胰液的分泌,增加其中脂肪酶分泌的总量,加强成年鹅对脂肪的消化,满足在半胱胺调节下快速生长对营养物质消化和能量的需求。
关键词:  半胱胺    胰液  胰脂肪酶
DOI:
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基金项目:国家自然科学基金资助项目(39570536)
Effect of cysteamine on the pancreatic secretion and lipase activity of geese
Abstract:
Twelve adult cross bred (Sichuan White × Tai Lake) geese were fitted with chronic pancreatic and duodenal cannulas.The birds were fed in daytime (8:00-20:00)ad libitum,whereas fasted in nighttime.The experiment consists of control and treated phase.Cysteamine (CS) was added in the diet at the dosage of 100 mg/kg·BW on the first day of treated phase.The pancreatic juice samples were collected continuously for three control days and eight treated days.The results showed that:① CS increased the average rate of pancreatic secretion by 71.28%-399.77%(P<0.01),in which that of daytime was elevated by 48.0%-456.09%(P<0.01),while that of nighttime elevated by 143.74%-311.42% (P<0.01).The secretion volume at daytime was more than that of night.② CS made the average lipase activity lower than that of control (P>0.05),the daytime lipase activity was higher than that of control in 3-5 days and the 7th day (P>0.05),whereas that of nighttime was significant lower (P<0.05).③ The total activity of lipase per hour was higher than that of control in day 1-5 (P<0.01),during which the total activity in daytime was greater than those in nighttime.These results indicate that CS can affect the pancreatic juice secretion and lipase activity by depleting the somatostatin,so it can improve the digestive foundation and supply more nutrition for quick growth in geese.
Key words:  cysteamine  geese  pancreatic secetion  pancreatic lipase