| 摘要: |
| 将200只1日龄健康雏鸡随机分为6组，第1～5组分别用H120,H52和IB二价油佐剂灭活苗进行单独或多重组合的免疫接种，于接种后每隔7d测1次HI抗体，并于第73天用肾型IBV强毒攻击，观察各组的保护率，第6组不免疫作为对照，结果显示，H120弱毒苗单独免疫组，H120＋IB二价油佐剂灭活苗组，H120＋H52弱毒苗组，IB二价油苗1次免疫组和IB二价油苗2次免疫组的保护率介绍为57.1％，85.8％，67％，83.3％和80％，非免疫对照组保护率为50％，试验结果表明，H120与H52弱毒苗对肾型HBV的保护力弱，H120＋IB二价油佐剂灭活疫苗与IB二价油佐剂灭活苗1次和2次免疫均可获得对肾型IBV较高的保护率，且H120与IB二价油佐剂灭活苗同时免疫可获得长时间的保护性抗体。 |
| 关键词: 鸡传染性支气管炎 弱毒疫苗 油佐剂灭活疫苗 HI抗体 |
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| 基金项目:杨陵农业科技开发基金项目(96JK-13) |
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| Multi-vaccinated study of chickens inoculated with avian infectious bronchitis vaccines |
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| Abstract: |
| 200 one-day-old chicks were divided into 6 groups.Group 1-5 were inoculated lonely or multiplely with Avian Infectious Bronchitis(IB) vaccines which including attenuated Virus of H120 and H52 strain as well as bivalent oil emulsion inactivated Vaccine(OEV).Group 6 was not inoculated as control test.Anti-IBV antibody titers of the 6 groups of chickens were tested by hemagglutination inhibition (HI) test once a week after inoculation.Chickens were challenged with virulent IBV at the 73th day after inoculation.The protective rates of the 6 groups were observed.The results showed that the protective rates of H120 lonely inoculated group,H120 +IB OEV group,H120 +H52 group,IB OEV inoculated one time and two times group as well as control group were 57.1%,85.8%,67%,83.3%,80% and 50%,respectively.The protective rate of H120 and H52 strain was lower than IB OEV.Although chickens inoculated with H120 +IB OEV,IB OEV one time and two times all got higher protective rate,chickens inoculated with H120 strain and IB OEV simultaneously could get longer protective period than the other two groups. |
| Key words: Avian infectious bronchitis attenuated vaccine oil emulsion inactivated vaccine HI antibody |
