| 摘要: |
| 研究了用3种不同方法（A法：程序冷冻；B法：超快速冷冻；C法：玻璃化冷冻）冷冻保存的牛GV期卵母细胞的发育潜力。结果表明，解 冻后，C组卵母细胞形态正常率显著高于A、B组；3种方法冷冻的卵母细胞成熟培养后，平均卵丘扩展率为65％,其中C组最高（69％）；3组冷冻卵母细胞的体外成熟率、体外受精率和卵裂率分别为14.3％,17.1％,21.6％；11.1％,12.6％,15.1％和5.6％,9.1％,11.1％。结果还表明：玻璃化冷冻的卵母细胞损伤较轻。 |
| 关键词: 牛 卵泡卵母细胞 冷冻保存 体外受精 |
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| 基金项目:山东省自然科学基金资助项目(Q99D06) |
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| Research on cryopreservation of bovine follicular oocytes |
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| Abstract: |
| The developmental competences of bovine GV-Stage oocytes cryopreserved by three methods (Method A:Conventional freezing procedures;Method B:ultrarapid freezing;Method C:Vitrification) were studied.After thawing,the morphological normal rate of C gronp was significantly higher than those of group A and B.The average cumulus expansion rate of all the cultured oocytes was 65% and that of group C was the highest(69%).The in vitro maturation rates were 14.3%,17.1% and 21.6%,and the fertilization rates were 11.1%,12.5% and 15.0%,and the oocyte cleavage rates were 5.6%,9.1% and 11.1% in group A,B,C oocytes cryopreserved respectively.The results showed that the injuries of vitrified oocytes were less severe. |
| Key words: bovine follicular oocytes cryopreservation in vitro fertilization |
