| 摘要: |
| 人工设计并合成了抗菌肽B基因的4个寡聚核苷酸片段，通过重叠区扩增法，扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。经克隆测序，证明成功地实现了抗菌肽基因的人工合成、拼接和克隆。 |
| 关键词: 重叠区扩增法 抗菌肽 基因合成 基因克隆 |
| DOI： |
| 分类号:S153.3 |
| 基金项目:北京市科委合同项目(855130400) |
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| Synthesis and cloning of antibacterial peptide B (cecropin B) gene by overlap extension |
|
YANG Ya-ti 1 ZHANG Yi-ping 2 ZHANG Xing-fu 2
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| Abstract: |
| Designed and synthesised 4 fragments of antibacterial peptide B gene,then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing).The interested fragment was cloned and sequenced and showed a good result as expected. |
| Key words: gene splicing by overlap extension antibacterial peptide gene synthesis gene cloning |
