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冬小麦芬蘖期氮素亏缺时间对某些生理特性的影响
曹翠玲1, 李生秀1, 李学俊2
1.西北农林科技大学 资源环境学院;2.西北农林科技大学 生命科学学院
摘要:
应用含1/9强度氮素的Hoagland营养液,对分蘖期小麦分别进行5,10和15 d的胁迫处理,以生长在正常Hoagland营养液中的小麦为对照,探讨了氮素胁迫时间对冬小麦叶绿素、硝酸还原酶活性(NRA)、可溶性蛋白、硝态氮、铵态氮以及根系活力的影响。结果表明,胁迫15 d后,叶绿素含量下降;硝酸还原酶活性和可溶性蛋白变化趋势基本一致;胁迫后根系硝酸还原酶活性均低于对照水平;叶片与根的硝态氮含量在胁迫后均低于对照水平,但变化趋势一致;铵态氮含量在胁迫后5,10 d高于对照水平;胁迫5d时根系活力和根系还原活性高于对照且达到最高值。
关键词:  氮素亏缺  胁迫时间  分蘖期  生理特性
DOI:
分类号:S152.7
基金项目:国家自然科学基金资助项目 (49890330,39770425和G1999-011707)
The effect of nitrogen stress time on some physiologic characteristics in winter wheat tillering stage
CAO Hong-xia  KANG Shao-zhong  WU Hai-xia
Abstract:
Nutrient solution culture was used to study the effect of nitrogen stress time on the characteristic of wheat in tillering stage.The results showed that after stressing for 15 d,the content of chlorophyll decreased;the changing trend of NRA and content of soluble protein was the same;after N stress,NRA of root was lower than that of the control,the content of nitrate and soluble protein were lowered;after stressing for 5 d and 10 d,content of ammonia increased;stressing for 5 d,the activity of root and activity of root redox increased too.
Key words:  nitrogen deficiency  stress time  wheat tillering stage  physiologic characteristic