| 摘要: |
| 利用原生质体融合技术，以链霉素和青霉素分别作为苜蓿中华根瘤菌（Sinorhizobium meliloti)102F28和鹰嘴豆中慢生根瘤菌（Mesorhizobium ciceri)USDA3383的抗药性选择标记，成功地获得了102F28和USDA3383的属间融合子。该融合子可分别在双亲寄主植物上结瘤，其在细胞形态、大小和蛋白质电泳图谱上与亲本菌株均有所差异。融合子与102F28的DNA同源性为90.8%，而与USDA3383的DNA同源性为15.2%。 |
| 关键词: 原生质体融合 苜蓿中华根瘤菌 鹰嘴豆中慢生根瘤菌 |
| DOI： |
| 分类号:S154.4 S152.1 |
| 基金项目:国家自然科学基金资助项目(3000005) |
|
| Study on fusion of protoplasts from Sinorhizobium meliloti and Mesorhizobium ciceri |
|
ZHAO Li ming CHEN Hao
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| Abstract: |
| Streptomycin and penicillin were regarded as the sign of resistance to antibiotics of Sinorhizobium meliloti 102F28 and Mesorhizobium ciceri USDA3383 respectively.Using the protoplast fusion technique,102F28 and USDA3383 were successfully fused.Fusion hybrid can inoculate in the leguminous of parental strains respectively.There were apparent differences between parents and fusion hybrid in cell morphology,and pattern of whole-cell protein.The values of DNA homology between fusion hybrid and 102F28 and USDA3383 were 90.8% and 15.2% respectively. |
| Key words: Protoplast fusion Sinorhizobium meliloti Mesorhizobium ciceri |
