| 摘要: |
| 将构建的绵羊β-乳球蛋白基因(BLG)调控人胰岛素原基因的重组基因通过显微注射生产转基因小鼠。共移植888枚注射BLG-胰岛素原基因的小鼠卵,移植受体31只,怀孕14只,产仔53只。PCR检测51只,有5只为阳性,并均为雌性,其中2只小鼠泌乳,经放免检测小鼠乳汁中人胰岛素原的质量浓度分别为37.44mg/L和39.99mg/L。另外3只异常消瘦而不孕,其中1只极度衰竭而死亡。 |
| 关键词: 人胰岛素原基因 转基因小鼠 基因表达 |
| DOI： |
| 分类号: |
| 基金项目:国家863计划资助项目(Z21-03-03) |
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| Expression of human proinsulin gene in milk of transgenic mice |
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HUANG Jun cheng SHI Hong cai LI Wen rong WU Jian YANG Dong mei GUO Zhi qin
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| Abstract: |
| The recombinant gene containing human proinsulin gene driven by ovine β-lactoglobulin gene was microinjected into the pronucleus of mouse zygotes.A total of 888 eggs injected were transferred into 31 recipients, of which 14 recipients were pregnant and produced 53 pups, and there were 5 transgenic female mice in 51 pups screened by PCR.Milk samples, collected from two lactating transgenic mice, contained 37.44 mg/L and 39.99 mg/L human proinsulin respectively by radioimmunoassay.Other three transgenic mice were too weak to be pregnant, of which one was dead. |
| Key words: Human proinsulin gene transgenic mice gene expression |
