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地锦草体外抑菌有效部位的筛选试验
宋晓平1, 王晶钰1, 李引乾1
西北农业大学 动物科学与动物医学学院
摘要:
采用水煎、水醇和五部位提取法从地锦草中提得组成成分不同的7种提取物,以肠道致病性大肠杆菌O78∶K80,沙门氏菌C79-1,金黄色葡萄球菌为目的菌株进行体外抑菌试验。结果表明,水煎提取物对大肠杆菌O78∶K80,沙门氏菌C79-1,金黄色葡萄球菌的最小抑菌质量浓度分别为10, 10, 12. 5 g /L;水醇提取物对上述试验菌的最小抑菌质量浓度依次为5, 7. 5, 10 g /L;五部位法所得5种提取物的抑菌浓度均大于15 g /L。
关键词:  地锦草,体外抑菌,有效部位
DOI:
分类号:S853.74
基金项目:西北农业大学中兽医药研究所资助项目
Selection of bacteriostasis positions of Euphorbia humifusa wild in vitro
Abstract:
Seven kinds of extract were obtained from Euphorbia humifusa Wild by water decoction, water ethanol and five position methods, and were conducted bacteriostasis test with intestine pathogenic Colon bacillus O78∶K80, Salmonella Pullorum C79-1, staphylococcus aureus for target bacteria in vitro. The results indicated that the minimum concentration of bacteriostasis of water decoction extract for Colon bacillus O78∶K80,Salmonella Pullorum C79-1 staphylococcus aureus was 10, 10, 12. 5 g/L crude drug, respectively. The minimum concentration of bacteriostasis of water ethanol extract on abive-nebtuibed testbacteria was 5, 7. 5, 10 g/L crude drug, successively.The concentration of bacteriostasis of five kinds of extract which obtained by five position methodwas higher than 15 g/L crude drug.
Key words:  Euphorbia humifusa wild,bacteriostasis in vitro,effective position