| 摘要: |
| 将采自渭北烟田的黄瓜花叶病毒(CMV)分离纯化后,制备抗血清并建立了双抗体夹心式酶联免疫检测方法(DAS-ELISA),同时对其各项指标作了合理选择,结果发现最适包被和酶标抗体的质量浓度均为0. 625 mg·L-1.用此方法测定表明,在渭北烟区越冬油菜是CMV的主要越冬寄主;CMV侵染烟草所引起的花叶病约占64. 3%。 |
| 关键词: 黄瓜花叶病毒 烟草 酶联免疫检测方法 |
| DOI： |
| 分类号:S435.72 |
| 基金项目:陕西省科委资助项目 |
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| The Establishment of ELISA for the Detection of Cucumber Mosaic Virus in Weibei Tobacco Planting Area of Shaanxi Province |
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| Abstract: |
| The cucumber mosaic virus (CMV) was isolated from tobacco leaves from Weibei tobacco-planting area,multiplicated in special hosts and purified.The purified virus was used to immunize rabbits to produce antiserum and established the DAS-ELISA detection system.The concentrations of coating and enzyme-labeled antibody were all 0.625 mg.L-1,and the standard detection curve was drawn.Using this ELISA detection system for the detection of CMV from tobacco plants and overwintering hosts,it were concluded that the tobacco mosaic disease induced by CMV was 64.3% and that rape was the most important primary inoculum source of CMV in this area. |
| Key words: cucumber mosaic virus,tobacco,ELISA detection |
