| 摘要: |
| 分别用70mL·L-1乙醇2m in和55 V·mm-1电场640μs一次脉冲,对注射hCG 15 h的小鼠卵母细胞作激活处理。结果表明:虽然乙醇处理的活化率(55. 40% )略高于电刺激(46. 90% ),但二者没有显著差异;与乙醇比较,电激活的卵母细胞能保持良好的发育能力,二者的桑椹胚发育率分别为31. 51%和91. 37%,差异极显著(P< 0. 01).说明电场作用后卵母细胞的活动更符合其正常的活化规律。 |
| 关键词: 卵母细胞，孤雌发育，小鼠 |
| DOI： |
| 分类号:S823.36, S814.6 |
| 基金项目:国家自然科学基金资助项目（39570526） |
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| Activation of the Early Mouse Oocytes by Alcohol and Electricity |
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| Abstract: |
| The different responses of early mouse oocytes to alcoholic and electrical
treatment were investigated in this study.Mouse oocytes, collected from superovulated
mouse 15 h after hCG injection,were stimulated by 70mL·L-1 alcohol (in PBS) 2min
or a 55 V·mm-1 640μs electrical pulse respectively. The results showed that, there
were 55. 40% oocytes actived after alcohol treatment, and the same numberwas 46. 90%
in electric stimulated oocytes.They had no significant differences (P>0. 05).Many actived oocytes include two pronuclear, and those oocytes with two PN develop much better. In the level of morulae rate, it can be found that electric stimulation can cause higer
development ability than alcohol (91. 37%∶31. 51%,P< 0. 01). The results suggest
that electric stimulation be a more efficent method to active early mouse ocytes.The possible reasonswere also discussed. |
| Key words: oocyte,activation,mouse |
