| 摘要: |
| 研究了FCS, 17β-E2,FSH, LH对牛卵泡卵母细胞成熟的作用及培养时间对卵母细胞核和细胞质成熟的影响。结果表明,添加体积分数为10%～15%的FCS, 1～2mg·L-117β-E2, 20～50 mg·L-1LH和10 mg·L-1FSH有利于提高卵母细胞核成熟,放出第一极体。而成熟培养20, 24 h的卵母细胞成熟率显著高于培养16 h(P<0. 05),核成熟后培养3～4 h有利于卵母细胞质成熟,对其后的受精、卵裂及胚胎的进一步发育有重大影响。且TCM 199+质量分数10%～15%的FCS+ 1～2mg·L-117β-E2+ 10mg·L-1FSH+ 20～50mg·L-1LH是卵母细胞成熟的理想培养系统。 |
| 关键词: 牛，体外成熟，卵母细胞 |
| DOI： |
| 分类号:W823.36, Q492.5 |
| 基金项目:农业部生物技术重点项目（95牧-01-04-01） |
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| Studies on Bovine Oocytes in Vitro Maturation |
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| Abstract: |
| The effects of FCS, culture time, 17β-estradial, FSH and LH on the
bovine oocytes in vitromaturation were studied.The results suggested that addtions of
10%～15% FCS, 1～2mg·L-117β-E2, 20～50mg·L-1LH and 10mg·L-1FSH be
effective to improve oocyte nuclear maturation, and to extrude the first polar body.The
maturation rate ofoocytes cultured for 20, 24 h was significantly higher than that of 16h
(P<0. 05).After nuclear maturation, culture for 3～4 h was benefical to oocyte cyto-
plasm maturation, and had a significant effect on fertilization, cleavage and embryo development.Addition of 10%～15% FCS, 10mg·L-1FSH 10～50mg·L-1LH and 1～2mg·L-117β-E2 in the TCM 199 medium were an idealculture system for bovine oocyte in vitro maturation. |
| Key words: bovine,in vitro maturation,oocyte |
