| 摘要: |
| 采用H2O∶CH2Cl2萃取、732型阳离子树脂交换等方法,从豆类丝核菌中分离到纯结晶,经薄板层析法发现,豆类丝核菌提取物浓缩的粗品液和苦马豆素标准品经Ehrlichs试剂显色后,二者均出现一椭圆形紫色斑点,且Rf值均为0. 81;气相色谱法研究发现,提取物纯品标样(15. 942 0mg/L)与苦马豆素标准品在同一色谱条件下显示出完全一致的峰形,证明从豆类丝核菌中分离到的纯结晶是苦马豆素纯品。研究还表明豆类丝核菌干燥菌丝体中苦马豆素含量达1. 23%～2. 99%. |
| 关键词: 豆类丝核菌，苦马豆素，提取与分离 |
| DOI： |
| 分类号:R284 |
| 基金项目:杨凌科研基金资助项目（93J-02）;西北农业大学青年科研基金资助项目 |
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| Abstraction of Swainsonine from Rhizoctonia leguminicola |
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| Abstract: |
| The crystals of swainsonine were isolated from Rhizoctonia leguminicola
by means of extraction between H2O∶CH2Cl2 and a 732 type cation exchang resin for
the first time in China. It discoverd that the purple elliptic spots are displayed in thinlayer chromatography (TCL) between semifinished product solution of donse abstracts
of R.dleguminicola and standardized sample of swainsonine dyed with Ehrlich′s reagent
and all the volumes of Rf are 0. 81.The study with gas chromatography (GC) discovers
that the same peaks type appear in pure abstract sample (15. 942 0mg/L) and standardized sample of swainsonine under the same conditions, and therefore it is clear that the
pure crystals isolated from R.leguminicola is pure swainsonine. The studies show that
dry mycelium of Rhizoctonia leguminicola contains 1. 23% -2. 99% swainsonine,which
has opened a new way to obtain swainsonine. |
| Key words: Rhizoctionia leguminicola,swainsonine,Abstraction, |
