| 摘要: |
| 报道了将Dr Marc Zabeau的AFLP(Amplified Fragment Length Polymor-phism)技术中的DNA提取方法用于以PCR(Polymerase Chain Reaction)技术鉴定转基因植物——拟南芥、烟草和芜菁中。该方法需约1 h可完成植物DNA的提取,样品用量仅为30～100mg,产量可达30～80μg/g.粗提DNA(溶于10μL TE缓冲液)不必经过纯化,用TE缓冲液稀释5～10倍即可直接用于PCR分析。在拟南芥、烟草和芜菁转基因植物鉴定中应用表明这一方法是目前以PCR法鉴定转基因植株T1代单株幼苗的一种简便、快速、有效的方法。 |
| 关键词: DNA提取方法，PCR分析，转基因植株鉴定，拟南芥，烟草、芜菁 |
| DOI： |
| 分类号:S603.5 |
| 基金项目: |
|
| A New Method of DNA Extraction for Identification of Transgenic Plants with PCR |
|
Gong Zhenhui 1 Cecchini E 2 Milner J J 2
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| Abstract: |
| A DNA isolation method developed by DrM arc Zabeau for the AFLP
(Arabidopsis thaliana,Nicotiana tabacum) technology to identify transgenic plants
(Arabidopsis thaliana,Nicotiana tabacumand turnip)with PCR was reported.The total
time for the extraction procedure of the method was approximately an hour. Samples
(rosette leaves or tissue) needed were only 30～100mg and DNA yield could reached 30
～80μg/g.The crude isolation DNA (dissolved in 10μL of TE buffer) does notneed to
be purified and could be directly used to PCR analysis after being diluted to 5～10
times.The application shows this method is convenient, quick and effective in identifying
individual T1 seedling of transgenic plants with PCR. |
| Key words: DNA extraction,PCR assay,identifing transgenic plant,Arabidopsis thaliana,Nicotiana tabacum,turnip |
