摘要: |
比较山梨醇和蔗糖两种碳源在苹果微体繁殖中的作用表明,二者对增殖和生长的最适浓度均为30g/L.山梨醇代替蔗糖作碳源时芽的增殖倍数高,而新梢生长差,叶/茎值小。总碳源为30g/L时,采用不同比例的山梨醇和蔗糖配合有利于芽的增殖和生长。山梨醇对生根的作用显著低于蔗糖,而在山梨醇培养基上继代4次后,生根得到明显改善 |
关键词: 苹果,山梨醇,微体繁殖,增殖,生根 |
DOI: |
分类号:S661.103.5 |
基金项目: |
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Effect of Sorbitol as Carbon Source on Micropropagation of Apple |
Ma Fengwang Wang Fei
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Abstract: |
The influences of two carbon source,sorbitol and sucrose,in micropropa-gation of apple were compared.The results showed that the optimal concentration ofboth sorbitol and sucrose was 30 g/L for shoot proliferation and growth.The substitu-tion of sorbitol for sucrose could promote proliferation,but could decrease shoot growthleaf/stem ratio,A medium with various proportion with total carbon source of 30 g/L of sorbitol and suerose favored both proliferation and shoot growth.Rooting was worse onsorbitol medium than on sucrose one but would be greatly enhanced after subcultured 4 times. |
Key words: apple,sorbitol,micropropagation,proliferation,rooting, |