| 摘要: |
| 比较山梨醇和蔗糖两种碳源在苹果微体繁殖中的作用表明，二者对增殖和生长的最适浓度均为30g/L．山梨醇代替蔗糖作碳源时芽的增殖倍数高，而新梢生长差，叶/茎值小。总碳源为30g/L时，采用不同比例的山梨醇和蔗糖配合有利于芽的增殖和生长。山梨醇对生根的作用显著低于蔗糖，而在山梨醇培养基上继代4次后，生根得到明显改善 |
| 关键词: 苹果，山梨醇，微体繁殖，增殖，生根 |
| DOI： |
| 分类号:S661.103.5 |
| 基金项目: |
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| Effect of Sorbitol as Carbon Source on Micropropagation of Apple |
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Ma Fengwang,Wang Fei,Ma Fengwang,Wang Fei
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| Abstract: |
| The influences of two carbon source,sorbitol and sucrose,in micropropa-gation of apple were compared.The results showed that the optimal concentration ofboth sorbitol and sucrose was 30 g/L for shoot proliferation and growth.The substitu-tion of sorbitol for sucrose could promote proliferation,but could decrease shoot growthleaf/stem ratio,A medium with various proportion with total carbon source of 30 g/L of sorbitol and suerose favored both proliferation and shoot growth.Rooting was worse onsorbitol medium than on sucrose one but would be greatly enhanced after subcultured 4 times. |
| Key words: apple,sorbitol,micropropagation,proliferation,rooting, |
