| 摘要: |
| 以辣椒无菌苗叶片为外植体，就激素对其不定芽分化及植株再生的影响进行了研究。结果表明，BA对叶片不定芽的诱导效果最好，ZT次之，KT不能有效地诱导叶片形成不定芽；IAA与BA相配合使叶片不定芽分化频率明显提高；IBA或NAA与BA的激素组合使叶片不定芽分化频率有所不降；2，4－D对叶片不定芽分化有明显的抑制作用。在分化培养基中添加1.0mg／L GA3有利于不定芽的伸长。不定芽伸长后在不含激素的MS或MS＋MA培养基上诱导生根，形成完整植株。 |
| 关键词: 辣椒，叶片离体培养，植物激素，植株再生 |
| DOI： |
| 分类号:S641.303.5 |
| 基金项目:国家博士点基金 |
|
| In Vitro Plant Regeneration from Leaf Explants of Pepper(Capsicum annuum L.) |
|
He Xiaoming Wang Ming Wang Zhezhi
|
| Abstract: |
| Effects of hormones on differentiation of adventitious buds and plant regeneration were studied in leaf culture of pepper in vitro.BA was considered as the best growth regulator for adventitious bud differentiation within 9 cytokinins,The combination of IAA and BA raised the differentiation frequency obviously,Thp highest differentiation frequency of adventitious buds was obtained in the medium MS BA 6.0mg/L IAA 1.0mg/L,while the lower ones were observed in the combinations of IBA or NAA with BA.2,4-D,however,showed the inhlbition effect on the bud differentiation.Adding GA3 in differentiation media promoted the elongation of adventitious buds,The elongated shoots rooted in media MS without hormone or MS IAA,and developed into complete plants. |
| Key words: pepper(Capsicum annuum),leaf culture,hormone effect,plant regeneration |
