| 摘要: |
| 本文研究了影响毛樱桃(Prunus tomentosa Thunb.)茎尖培养的有关因素。毛樱桃茎尖在 MS,G,L 三种培养基上均可增殖生长,但以在 L 培养基中生长较好。在附加的六类激素中,以IBA,BA 最为有效,适宜的浓度为 IBA 1.0,BA 1.0或 ZT 1.0。诱导生根以1/2培养基附加 NAA 0.1较好,削伤和暗培养可大大提高生根率。移栽入土前,在0.1%克菌丹溶液中浸根5分钟可提高成活率。 |
| 关键词: 毛樱桃,茎尖培养,增殖生长,生根率,成活率 |
| DOI： |
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| A Preliminary Research on Shoot Tip Culture of Manchu Cherry (Prunus tomentosa Thunb.) in Vitro |
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Dong Yihu Yang Zenghai Hu Niyun Lu Guangming
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| Abstract: |
| This paper deals with some factors affecting shoot tip culture of Manchu cherry(Prunus tomentosa Thunb.)in vitro.Manchu cherry shoot tips can propagate on such three kinds of mediums as MS,G and L mediums,growing vigorously on L medium.In the additional hormones of six kinds,IBA ana BA are the most effective ones with the optimum concentration of IBA 1.0,BA 1.0 or ZT 1.0.?L medium supplemented with NAA 0.1 is better for the induction of shoot propagation.The rooting rate can be greatly improved by a basal wound on one or two sides of the shoot base and incubation in the dark.prior to transplanting,the roots of plantlets should be soaked in Captan solution with a concentration of 0.1% for 5 minutes so that the survival rate may be increasingly raised. |
| Key words: Manchu cherry (Prunus tomentosa Thunb),shout tip culture in vitro growing vigorously,rooting rate,survival rate |
