| 摘要: |
| 【目的】阐明牡丹花瓣发育过程中细胞增殖与细胞扩张转换的细胞学进程与分子调控网络，为牡丹花 瓣发育障碍的消除调控提供理论依据。【方法】以牡丹‘户川寒’为材料，采集春季开花过程中8个花瓣发育关键时期 样品，结合形态学观测、转录组测序、代谢组检测及多组学联合分析，解析牡丹花瓣发育调控网络。【结果】①牡丹花 瓣发育可分为细胞增殖期、转换期和扩张期3个阶段。花瓣面积达到最终大小约10%时，可能是花瓣细胞增殖向细胞 扩张转换的关键节点；细胞数量增长主导增殖期，而细胞伸长是扩张期的主要驱动力。②生长素、细胞分裂素、赤霉素和脱落酸含量在不同花瓣发育阶段呈现动态变化，可能参与发育时序调控。③筛选到PsCKX3、PsGAI、PsARF2、 PsEXPA11等12个在花瓣发育时序上呈现特异性表达模式、具有作为发育阶段标志基因潜力的核心候选基因，初步构建了以激素信号转导为核心、整合细胞周期调控与细胞扩张相关基因的层级调控网络框架。【结论】牡丹花瓣形态 发育依赖细胞增殖和扩张，花瓣面积达到约终态面积10%时为转换关键节点。植物激素通过调控细胞周期与扩张相 关基因的表达，协同介导花瓣发育进程的时序转换。 |
| 关键词: 牡丹 花瓣发育 细胞增殖 细胞扩张 植物激素 转录组 RT-qPCR |
| DOI：10. 13207/j. jnwafu. 2027. 01. 007 |
| 分类号: |
| 基金项目:国家林业和草原局行业标准项目（牡丹综合体（2018-LY-054））；北京林业大学建设世界一流学科和特色发展引导专项 （2019XKJS0324） |
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| Multi-omics analysis the transition mechanism between cell proliferation and expansion during petal development in tree peony |
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Yuan Xue1,2, Yuan Tao1, Liu Shaodan3
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1.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education，Beijing Laboratory of Urban and Rural Ecological Environment/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular
Breeding/National Engineering Research Center for Floriculture，School of Landscape Architecture，Beijing Forestry University，Beijing 100083，China;2.Southwest Guizhou Vocational and Technical College for Nationalities，Xingyi，Guizhou 562400，China;3.Luoyang International Peony Garden，Luoyang，Henan 471011，China
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| Abstract: |
| 【Objective】This study aims to clarify the cytological processes and molecular regulatory net-work underlying the transition from cell proliferation to cell expansion during the petal development of tree peony （Paeonia×suffruticosa），and to provide a theoretical basis for the regulation of petal developmental disorders.【Method】Using tree peony‘ Yusenkan’ as the material，samples were collected at eight key stages of petal de-velopment during spring flowering. Through the integration of morphological observation，transcriptome se-quencing，metabolome profiling，and multi-omics analysis，we aimed to elucidate the underlying regulatory net-work.【Result】① Petal development of tree pony could be divided into three phases：the cell proliferation phase，the transition phase，and the cell expansion phase. The stage at which the tree peony petal area reached approxi-mately 10% of its final size might be the key node for the proliferation-to-expansion transition. Increase in cell number dominated the proliferation phase，whereas cell elongation was the main driver of the expansion phase. ②The content levels of auxin，cytokinin，gibberellin，and abscisic acid exhibited dynamic changes across different stages，and may be involved in the sequential regulation of development.③ Twelve core candidate genes，inclu-ding PsCKX3，PsGAI，PsARF2，and PsEXPA11，were identified，which exhibited stage-specific expression pa-tterns
during petal development and had the potential to serve as marker genes for developmental phases. A pre-liminary hierarchical regulatory network framework was established，which centered on hormone signal transduc-tion and integrated genes involved in cell cycle regulation and cell expansion.【Conclusion】The morphogenesis of tree peony petals relies on both cell proliferation and cell expansion，with the petal area reaching about 10% of the final size，serving as a critical transition node between these two processes. Phytohormones coordinately me-diate the temporal transition of petal development by regulating the expression of genes related to the cell cycle and cell expansion. |
| Key words: Paeonia×suffruticosa petal development cell proliferation cell expansion phytohormone transcriptome RT-qPCR |
