| 摘要: |
| 【目的】对刺毛杜鹃不同花色的代谢通路及关键代谢产物变化进行研究，以探明刺毛杜鹃花色差异的代谢机理，为刺毛杜鹃花色改良和分子育种提供科学参考。【方法】以14年生盆栽刺毛杜鹃为材料，采用超高效液相色谱-质谱（UPLC-MS/MS）技术对白色（C-5）、浅红色（C-3）和深红色（C-1）刺毛杜鹃花瓣的花青素进行测定，在此基础上，采用靶向代谢组学筛选不同花色刺毛杜鹃差异代谢物，并采用京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）数据库对筛选得到的差异代谢物进行富集通路分析。【结果】在3种花色刺毛杜鹃花瓣中共检测到83种花青素物质，主要包含19种矢车菊素，15种飞燕草素，14种天竺葵素，13种矮牵牛素，8种芍药花素，6种锦葵色素，6种原花青素，槲皮素 、柚皮素各1种。 C-1_vs_C-3、C-1_vs_C-5和C-3_vs_C-5组中显著差异代谢物分别有24，43和38种，3个比较组共有的显著差异代谢物质有6种。KEGG通路注释发现，差异代谢物质在花青素生物合成通路中明显富集。【结论】锦葵色素-3-O-阿拉伯糖苷和锦葵色素-3-O-半乳糖苷是刺毛杜鹃花紫色调差异产生的关键代谢物，而芍药花素-3-O-阿拉伯糖苷是红色调差异产生的关键代谢物。 |
| 关键词: 刺毛杜鹃 代谢组学 代谢通路 花青素 代谢差异 |
| DOI：10. 13207/j. jnwafu. 2026. 10. 013 |
| 分类号: |
| 基金项目:浙江省科技计划省属科研院所扶持专项（2024F1065-4）；浙江省农业新品种重大专项花卉育种专项（2021C02071-2） |
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| Targeted metabolomics study of anthocyanins in the petals of Rhododendrons championae with different flower colors |
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YANG Hua1,2, SONG Xuzhong1,2
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1.Zhejiang Academy of Forestry，Hangzhou，Zhejiang 310023，China;2.Zhejiang Key Laboratory of Forest Genetics and Breeding，Hangzhou，Zhejiang 310023，China
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| Abstract: |
| 【Objective】The metabolic pathways and key metabolite changes of different flower colors of R.championae were studied and the metabolic mechanism of flower color differences of R.championae was expounded，to provide certain scientific references for the subsequent flower color improvement and molecular breeding of R.championae.【Method】Taking 14-year-old potted R.championae as the material，the anthocya?nins in the petals of white（C-5），pink（C-3）and dark red（C-1）R.championae were determined by UPLC-MS/MS technology. On this basis，targeted metabolomics was used to screen the differential metabolites of R.championae among different flower colors. The enrichment pathway analysis was conducted on the screened differential metabolites by using the Kyoto encyclopedia of genes and genomes（KEGG）database.【Result】A total of 83 types of anthocyanin substances were detected in the three samples，primarily including 19 cyanidins，15 delphinidins，14 pelargonidins，13 petunidins，8 peonidins，6 malvidins，6 proanthocyanidins，1 quercetin and 1 naringenin. There were 24 significantly differentiated metabolites in the C-1_vs_C-3 group，43 in C-1_vs_C-5 group，and 38 in C-3_vs_C-5 group. And there were 6 significantly differentiated metabolites shared by the three comparison groups. KEGG pathway analysis revealed significant enrichment of differential metabolites in the anthocyanin biosynthesis pathway.【Conclusion】 Malvidin-3-O-arabinoside and malvidin-3-O-galactoside were identified as the key metabolite responsible for flower purple color variation and peonidin-3-O-arabinoside for
red color in R.championae. |
| Key words: Rhododendrons championae metabolomics metabolic pathway anthocyanin difference of metabolism |
