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| 外源富氢水对盐胁迫下辣椒幼苗生长的影响及生理机制 |
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郭浩,常斌川,刘梦瑶,吴雪,石玉,叶林,曹凯,张毅
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1.山西农业大学 园艺学院，山西 晋中 030801;2.江苏省农业科学院 农业设施与装备研究所/农业农村部 长江中下游设施农业工程重点实验室，江苏 南京 210014;3.宁夏大学 葡萄酒与园艺学院，宁夏 银川 750021
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| 摘要: |
| 【目 的】探究富氢水（HRW）对盐胁迫下辣椒幼苗生长和生理指标的影响，为辣椒的设施高效栽培提供理论依据。【方法】以‘杭椒 1 号’辣椒幼苗为材料，采用盆栽试验，设置对照（CK）、HRW、NaCl和NaCl+HRW 4个处理 ，探究HRW对盐胁迫下辣椒幼苗生长、光合荧光特性、抗氧化酶活性、活性氧（ROS）、细胞膜稳定性及渗透调节物质含量的影响。采用偏最小二乘回归模型（PLS）-变量重要性投影（VIP）分析HRW影响盐胁迫辣椒幼苗生理的关键调控因子。【结果】HRW可以有效缓解盐胁迫对辣椒幼苗的生长抑制，促进幼苗叶片叶绿素积累，提高光合作用能力，同时HRW可以提高辣椒幼苗叶片和根系的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性，并降低叶片和根系的丙二醛（MDA）含量、电解质外渗率（EL）、超氧阴离子（O-2·）和过氧化氢（H2O2）含量。此外 ，HRW处理可促进盐胁迫下辣椒幼苗叶片和根系的可溶性蛋白（SP）、脯氨酸（Pro）和可溶性糖（SS）的积累，恢复细胞渗透势。PLS-VIP结果表明，叶片SP是HRW缓解盐胁迫下辣椒幼苗生长的最关键调控因子。【结论】HRW对盐胁迫下辣椒幼苗的生长和生理指标有明显影响，HRW主要通过提高盐胁迫下辣椒幼苗的渗
透调节能力和抗氧化能力，改善光合作用，从而有效缓解盐胁迫对辣椒幼苗的损伤。 |
| 关键词: 辣椒幼苗 富氢水 盐胁迫 偏最小二乘回归模型 |
| DOI：10. 13207/j. jnwafu. 2026. 10. 011 |
| 分类号: |
| 基金项目:山西省现代农业产业技术体系建设专项资金资助项目（2025CYJSTX08）；宁夏回族自治区重点研发计划项目（2023BCF-01042） |
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| Effects of exogenous hydrogen⁃rich water on growth and physiological mechanisms in pepper seedlings under salt stress |
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GUO Hao1,2, CHANG Binchuan1, LIU Mengyao1,3, WU Xue2, SHI Yu1, YE Lin4, CAO Kai2, ZHANG Yi1
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1.College of Horticulture，Shanxi Agricultural University，Jinzhong，Shanxi 030801，China;2.Institute of Agricultural Facilities and Equipment，Jiangsu Academy of Agricultural Sciences/Key Laboratory of Facility Agriculture Engineering in the Middle and Lower Reaches of the Yangtze River，Ministry of Agriculture and Rural Affairs，Nanjing，Jiangsu 210014，China;3.Institute of Agricultural Facilities and Equipment，Jiangsu Academy of Agricultural Sciences/Key Laboratory of Facility Agriculture Engineering in the Middle and Lower Reac;4.College of Wine and Horticulture，Ningxia University，Yinchuan，Ningxia 750021，China
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| Abstract: |
| 【Objective】 The study aimed to investigate the effects of hydrogen-rich water （HRW） on growth and physiological indicators of pepper seedlings under salt stress，providing a theoretical basis for efficient protected cultivation.【Method】Using pot-grown seedlings of pepper cultivar‘Hangjiao No. 1’，we estab-lished four treatments：control（CK），HRW，NaCl（salt stress），and NaCl+HRW. We examined the effects of HRW on the growth，photosynthetic fluorescence characteristics，antioxidant enzyme activities，reactive oxygen species（ROS），cell membrane stability，and osmotic regulatory substance contents of pepper seedlings under salt stress. The partial least squares（PLS）model with variable importance for projection（VIP）analysis was applied to identify key regulatory factors in the physiological responses of hydrogen-rich water（HRW）-treated pepper seedlings under salt stress.【Result】HRW significantly alleviated salt-induced growth inhibition in pepper seedlings，enhancing chlorophyll accumulation and photosynthetic efficiency. It could increase the activities of superoxide dismutase（SOD），peroxidase（POD），catalase（CAT），and ascorbate peroxidase（APX）in the leaves and roots of pepper seedlings，while reducing the contents of malondialdehyde（MDA），electrolyte leakage rate（EL），superoxide anion（O-2·），and hydrogen peroxide（H<2O2）in the leaves and roots. In addition，
HRW treatment could promote the accumulation of soluble protein（SP），proline（Pro）and soluble sugar（SS） in the leaves and roots of pepper seedling under salt stress，and restore cell osmotic potential. PLS-VIP analysis identified leaf soluble protein（SP）as the most critical regulatory factor for hydrogen-rich water（HRW）in alleviating the growth inhibition of pepper seedlings under salt stress.【Conclusion】HRW significantly enhanced growth and physiological attributes in salt-stressed pepper seedlings. It effectively alleviated salt stress damage mainly by improving the osmotic adjustment ability and antioxidant capacity，thereby boosting photosynthetic efficiency of pepper seedlings. |
| Key words: pepper seedlings hydrogen-rich water salt stress partial least squares regression model |
