| 摘要: |
| 【 目的】探究夜间增补不同强度UV-A对银杏生长生理以及类黄酮代谢的影响，选择适宜的夜间补光光质及技术提高银杏药用经济价值。【 方法】以1年生半同胞金叶银杏与绿叶银杏盆栽实生苗为研究对象，分别设置对照组（白光，300 μmol/（m2·s））和试验组，其中试验组在白天正常照射结束后，夜间增补强度分别为15，30和45 μmol/（m2·s）390 nm的UV-A紫外光，测定其不同生长期生物量、矿质元素和初生代谢物含量、抗氧化酶和苯丙烷生物合成酶活性以及总黄酮含量，分析夜间增补不同光照强度UV-A对银杏生长及生理代谢的影响。【 结果】①在夜间增补30 μmol/（m2·s）UV-A处理下，与对照组相比，绿叶银杏地上部生物量在快速生长期显著增加55.57%，在封顶期 根冠比最小，为对照的64%，生长状况最佳；生物量与矿质元素含量呈显著正相关。②在夜间增补30 μmol/（m2·s）UV-A处理下，封顶期金叶和绿叶银杏叶片的可溶性蛋白含量显著升高，分别为对照组的2.02和1.90倍。在封顶期，金叶银杏和绿叶银杏分别在夜间增补30和45 μmol/（m2·s）处理下POD活性最高，相较于对照分别增长19.8%和18.4%，且均显著大于对照组。③苗木封顶期，绿叶银杏的苯丙烷生物合成酶活性和叶片总黄酮含量在夜间增补30 μmol/（m2·s）的UV-A处理下最高，其总黄酮含量是对照的2.07倍。④模糊隶属函数法综合评价结果显示，在夜间增 补30和45 μmol/（m2·s）处理下银杏植株的隶属度均值均较高，表明该处理下叶片黄酮药用价值最佳。【 结论】在白天正常照射条件下，夜间增补30和45 μmol/（m2·s）的UV-A最有利于银杏幼苗生长和叶片黄酮积累，提升药用价值。 |
| 关键词: 银杏 夜间增补UV-A 生理代谢 黄酮积累 |
| DOI：10. 13207/j. jnwafu. 2026. 08. 009 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目（31971689）；江苏省科技计划（资金）项目（BE2021367） |
|
| Effects of nighttime supplemental UV⁃A at different intensities on the growth physiology and flavonoid accumulation of Ginkgo biloba |
|
LIU Jiayi1, WANG Gaiping1, WANG Wuque1, LU Xiaochun1, LIU Yuhua2, XU Zhibiao3
|
|
1.College of Forestry and Grassland，Southern Modern Forestry Collaborative Innovation Center，Nanjing Forestry University， Nanjing，Jiangsu 210037，China;2.Institute of Modern Forestry Technology Innovation，Jiangsu Vocational College of Agriculture and Forestry，Jurong，Jiangsu 212499，China;3.Yancheng Forest Farm，Yancheng，Jiangsu 224057，China
|
| Abstract: |
| 【 Objective】The study aimed to investigate the effects of different intensities of nighttime supple?mental UV-A on the growth，physiology，and flavonoid metabolism of Ginkgo biloba，and select optimal nighttime supplemental light quality and techniques to enhance its medicinal and economic value.【Method】One-year-old half-sibling potted seedlings of golden-leaf G.biloba and green-leaf G.biloba were used as materials.The seedlings were divided into control and experimental groups. After normal daytime light exposure，the experimental groups were exposed to supplemental nighttime 390 nm UV-A radiation at three different intensities：15，30，and 45 μmol/（m2·s）. During different growth stages，measurements were taken of biomass，mineral elements，primary metabolites，antioxidant-related enzymes，phenylpropanoid biosynthesis-related enzymes，and total flavonoids. The effects of nighttime supplemental UV-A at different intensities on the growth and metabo-lism of G.biloba were analyzed.【 Result】①When supplemented with nighttime UV-A irradiation at an inten-sity of 30 μmol/（m2·s），the aboveground biomass of green-leaf G.biloba significantly increased by 55.57% during the rapid growth period compared with the control group. At the terminal bud set stage，the root-to-shoot ratio was at its lowest，64% of that in the control group，indicating the optimal growth conditions. The biomass showed significant postive correlation with the mineral elements content. ②Under supplemental nighttime UV-A treatments of 30 μmol/（m2·s），the soluble protein content in the leaves of golden- and green-leaf G.biloba at the terminal bud set stage significantly increased，reaching 2.02-fold and 1.90-fold that of the control，respec-tively. At this stage，golden-leaf G.biloba exhibited the highest POD activity under the 30 μmol/（m2·s） treat?ment，while green-leaf G.biloba showed the highest activity under the 45 μmol/（m2·s） treatment，with increases of 19.8% and 18.4% compared with the control，respectively. Both values were significantly higher than those of the control. ③During the terminal bud set stage，the phenylpropanoid biosynthesis enzyme activity and total flavonoid content in the leaves of green-leaf G. biloba reached their highest levels under supplemental night-time UV-A radiation of 30 μmol/（m2·s），with the total flavonoid content being 2.07 times that of the control. ④ A comprehensive evaluation using the fuzzy membership function method revealed that the mean membership de-grees of G.biloba plants were relatively high under supplemental nighttime UV-A treatments of 30 and 45 μmol/（m2·s），indicating that these treatments yielded the optimal medicinal value of leaf flavonoids.【Conclusion】Under normal daytime irradiation conditions，supplemental nighttime UV-A radiation at 30，45 μmol/（m2·s） is most conducive to the growth of G.biloba seedlings and the accumulation of flavonoids in their leaves，thereby enhancing their medicinal value. |
| Key words: Ginkgo biloba nighttime UV-A supplementation physiological metabolism flavonoid accumu‑lation |
