| 摘要: |
| 【 目的】探究赤霉素（gibberellin，GA）和褪黑素（melatonin，MT）对盐碱胁迫下紫斑牡丹幼苗生长及生 理特性的影响，筛选出能有效增强紫斑牡丹耐盐碱性的外源物质及其最适质量浓度，为合理利用植物激素改善紫斑牡 丹耐盐碱性提供理论依据。【 方法】以3年生紫斑牡丹‘玉壶冰心’实生苗为材料，采用盆栽试验，共设10个处理，分别 为：CK.蒸馏水空白对照；S.盐碱胁迫处理（50~150 mmol/L NaCl-NaHCO3，其中n（NaCl）∶n（NaHCO3）=1∶1）；SG1. 盐碱胁迫+50 mg/L GA；SG2. 盐碱胁迫+100 mg/L GA；SG3. 盐碱胁迫+150 mg/L GA；SG4. 盐碱胁迫+200 mg/L GA；SM1. 盐碱胁迫+50 μmol/L MT；SM2. 盐碱胁迫+100 μmol/L MT；SM3. 盐碱胁迫+200 μmol/L MT；SM4. 盐碱胁迫+400 μmol/L MT，测定不同处理紫斑牡丹幼苗生长指标、叶绿素含量、抗氧化酶活性和渗透调节物质含量，在此基础上对不同处理紫斑牡丹耐盐碱性进行了综合评价。【 结果】与CK相比，盐碱胁迫处理紫斑牡丹幼苗叶片萎蔫 发黄，生长明显被抑制；而喷施不同浓度GA和MT可缓解盐碱胁迫处理幼苗叶片枯萎黄化症状，显著改善幼苗株高、 叶长、叶宽、根冠比，提高叶片相对含水量，增强叶绿素和可溶性蛋白、可溶性糖、脯氨酸含量以及SOD、POD和CAT活性，减少膜脂过氧化产物MDA的积累。综合评价结果显示，不同处理紫斑牡丹‘玉壶冰心’幼苗耐盐性的总得分排 名依次为SM3>SG2>SG3>SM2>SM4>SG4>SG1>SM1>S。【结论】适宜浓度的GA和MT能增强盐碱胁迫下紫斑 牡丹幼苗抗氧化和渗透调节能力，缓解盐碱胁迫对幼苗的伤害，提高幼苗耐盐碱能力，其中以100 mg/L GA和200 μmol/L MT处理效果较佳。 |
| 关键词: 紫斑牡丹 盐碱胁迫 赤霉素 褪黑素 生长与生理特性 |
| DOI：10. 13207/j. jnwafu. 2026. 05. 009 |
| 分类号: |
| 基金项目:甘肃省牡丹工程研究中心项目（XZ20181120）；甘肃省科技支撑计划项目（1604NKCA053） |
|
| Effects of gibberellin and melatonin on growth and physiological characteristics of Paeonia rockii seedlings under saline⁃alkali stress |
|
TANG Hong, XUE Nana, TAO Xuanlin, WANG Runlong, LIU Jiamiao
|
|
College of Forestry，Gansu Agricultural University，Lanzhou，Gansu 730070，China
|
| Abstract: |
| 【 Objective】The study aims to explore the effects of gibberellin（GA） and melatonin（MT） on the growth and physiological characteristics of Paeonia rockii seedlings under saline-alkali stress，and identify the exogenous substances that can effectively enhance the saline-alkali tolerance of P. rockii and their optimal mass concentrations，thereby providing a theoretical foundation for enhancing the saline-alkali tolerance of P. rockii via rational phytohormone application.【Method】Using 3-year-old seedlings of P. rockii‘ Yuhubingxin’ as materials，a pot experiment was conducted under 10 treatments：CK. Distilled water blank control；S. Salinealkali stress treatment （50-150 mmol/L NaCl-NaHCO3，n（NaCl）∶n（NaHCO3）=1∶1）；SG1. Saline-alkali stress+50 mg/L GA；SG2. Saline-alkali stress+100 mg/L GA；SG3. Saline-alkali stress+150 mg/L GA；SG4.Saline-alkali stress+200 mg/L GA；SM1. Saline-alkali stress+50 μmol/L MT；SM2. Saline-alkali stress+100 μmol/L MT；SM3. Saline-alkali stress+200 μmol/L MT；SM4. Saline-alkali stress+400 μmol/L MT. Growth indicators，chlorophyll content，antioxidant enzyme activities，and osmolyte contents of P. rockii seedlings under different treatments were measured. Based on these indices，a comprehensive evaluation of the salt-alkali tolerance of P. rockii under different treatments was conducted.【Result】Compared with CK，P. rockii seedlings un-der saline-alkali stress showed leaf wilting and yellowing，with significantly inhibited growth. Foliar application of different concentrations of GA and MT alleviated leaf wilting and yellowing symptoms，significantly improved seedling plant height，leaf length，leaf width，root-shoot ratio，and increased leaf relative water content. Treated seedlings exhibited elevated contents of chlorophyll，soluble protein，soluble sugar，proline，and activities of SOD，POD and CAT，and reduced accumulation of membrane lipid peroxidation product MDA. Comprehensive evaluation ranked salt tolerance for P. rockii seedlings under different treatments as SM3>SG2>SG3>SM2>SM4>SG4>SG1>SM1>S.【Conclusion】Appropriate concentrations of GA and MT can enhance the antioxidant and osmotic adjustment capabilities of P. rockii seedlings under saline-alkali stress，alleviate the damage to seedlings caused by saline-alkali stress，and improve the salt-alkali tolerance of seedlings. Treatment with 100 mg/L GA and 200 μmol/L MT demonstrate superior efficacy. |
| Key words: Paeonia rockii saline-alkali stress gibberellin melatonin growth and physiological characteristics |
