| 摘要: |
| 【目的】以苹果转录因子NF-X1基因家族为研究对象，对其不同组织表达特性及对盐胁迫的响应规律进行研究，为进一步探索苹果砧木抗盐功能提供参考。【方法】以苹果砧木八棱海棠（Malus×robusta）为供试材料，利用生物信息学方法，对NF-X1基因家族成员进行鉴定、基本理化性质分析、亚细胞定位预测及系统进化分析；使用TBtools，对MdNF-X1基因家族成员的基因结构、保守结构域、保守基序、启动子顺式元件等进行可视化分析；采用实时荧光定量PCR（RT-qPCR）技术，检测MdNF-X1基因家族的组织表达特异性及盐胁迫下不同组织中的响应特性。【结果】苹果MdNF-X1基因家族有MdNF-X1-1、MdNF-X1-2、MdNF-X1-3、MdNF-X1-4共4个成员，编码蛋白分别含1 162，1 138，865和830个氨基酸，亚细胞定位预测均为细胞核内蛋白。基于氨基酸序列的系统进化分析显示，4个MdNF-X1成员可分为2个亚组，且均与葡萄NF-X1家族亲缘关系最近。蛋白功能域分析显示，MdNF-X1家族成员均含有NF-X1-zinc-finger保守结构域，属于锌指转录因子；顺式作用元件分析表明，MdNF-X1基因家族启动子中含有盐、干旱胁迫及脱落酸、生长素等响应元件。RT-qPCR检测显示，MdNF-XX1-1和MdNF-XX1-3在根中表达量较高，MdNF-XX1-2在根和叶中表达较高且表达量相近，MdNF-XX1-4在各个组织中表达量均较低；在盐胁迫处理下，4个MdNF-X1基因在根、茎、叶中对盐胁迫均有响应，其中以MdNF-X1-1基因在根系中对盐胁迫的响应最为显著，相对表达量高于MdNF-X1基因家族的其他成员。【结论】苹果砧木八棱海棠中的MdNF-X1-1基因在盐胁迫下显著诱导表达，表明其可能参与八棱海棠对盐胁迫的应答调控，为进一步揭示其在抗盐胁迫中的具体功能提供了依据。 |
| 关键词: 苹果砧木 八棱海棠 NF X1基因家族 盐胁迫 转录因子 |
| DOI：10.13207/j.jnwafu.2026.03.001 |
| 分类号: |
| 基金项目:国家自然科学基金项目(32372640);新疆苹果产业技术体系项目（XJLGCYJSTX04-2024-15） |
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| Analysis of the NF-X1 gene family in apple and its tissue expression pattern under salt stress |
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BAI Xueqi1, ZHOU Jia2, SHI Tianle2, LI Tao1, ZHANG Junke2, LI Xingliang2
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1.College of Forestry，Shanxi Agricultural University，Taigu，Shanxi 030801，China;2.Institute of Forestry and Pomology，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100093，China
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| Abstract: |
| 【Objective】The apple transcription factor NF-X1 gene family was used as the research object to study its expression characteristics in different tissues and the response pattern to salt stress,providing a reference for further research on the salt resistance function of apple rootstocks.【Method】Using the apple rootstock,Malus×robusta,as the test material,bioinformatics methods were used to identify members of the NF-X1 gene family,analyze the basic physicochemical properties,predict the subcellular localization,and perform the phylogeny analysis.The gene structures,conserved domains,conserved motifs,and promoter cis-elements of the MdNF-X1 gene family members were visualized using TBtools.Real-time quantitative PCR (RT-qPCR) was used to detect the tissue-specific expression patterns of the MdNF-X1 gene family and its response properties in different tissues under salt stress.【Result】The apple MdNF-X1 gene family comprises four members,MdNF-X1-1,MdNF-X1-2,MdNF-X1-3,and MdNF-X1-4,encoding proteins with 1 162,1 138,865 and 830 amino acids,respectively,all predicted to be nuclear in subcellular localization.Phylogenetic analysis based on amino acid sequences revealed that the four MdNF X1 members could be classified into two subgroups,all being most closely related to the grape NF-X1 family.Protein functional domain analysis showed that all MdNF-X1 family members contained the NF-X1-zinc-finger conserved domain,identifying them as zinc-finger transcription factors.Cis-acting element analysis demonstrated that the promoters of MdNF-X1 gene family contained response elements to salt stress,drought stress and response elements including abscisic acid and auxin. RT-qPCR analysis showed that MdNF-X1-1 and MdNF-X1-3 had higher expression in roots;NF-X1-2 had higher and similar expression in roots and leaves;MdNF-X1-4 had lower expression in all tissues. Under salt stress,all four MdNF-X1 genes responded in roots,stems,and leaves.Among them,MdNF-X1-1 exhibited the most significant response to salt stress in the roots,with its relative expression exceeding other family members.【Conclusion】The expression of MdNF-X1-1 gene in the apple rootstock Malus×robusta was significantly induced under salt stress,suggesting its involvement in the response regulation of Malus×robusta under salt stress.This provides a basis for further revealing its specific functions in salt stress resistance. |
| Key words: apple rootstock Malus×robusta NF X1 gene family salt stress transcription factor |
