| 摘要: |
| 【目的】克隆北苍术1-羟基-2-甲基-2-(-E-)-丁烯基-4-二磷酸还原酶基因（AcHDR），探究其生物学特征和不同组织及盐胁迫下的表达模式，为研究AcHDR功能提供理论依据。【方法】用RACE技术克隆AcHDR基因，分析AcHDR编码蛋白的理化性质、保守结构域、保守性及结构，构建系统发育树；用实时荧光定量PCR （RT-qPCR）技术检测AcHDR在不同组织及100 mmol/L NaCl胁迫处理不同时间下的表达量；测定不同盐胁迫下叶片的光合特性。【结果】AcHDR（GenBank登录号：PQ369427）开放阅读框全长为1 383 bp，编码460个氨基酸，具有lytB_ispH结构域，在进化过程中相对保守，与生菜、菊苣和橡胶草的HDR亲缘关系较近。RT-qPCR结果表明，AcHDR在北苍术叶中表达量最高；在100 mmol/L NaCl胁迫处理不同时间后，AcHDR表达量呈现先升高后降低的趋势。150 和250 mmol/L NaCl胁迫显著降低了北苍术光合特性。【结论】AcHDR基因快速响应盐胁迫，可能会提高植物的耐盐性。 |
| 关键词: 北苍术 HDR qRT-PCR RACE技术 |
| DOI：10.13207/j.cnki.jnwafu.2025.12.021 |
| 分类号: |
| 基金项目:河北省中央引导地方科技发展资金项目“自由探索类基础研究”(246Z2512G) |
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| Cloning and expression analysis of the HDR gene in Atractylodes chinensis (DC.) Koidz. |
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WANG Xiaoyu, LI Xiaohui, YAN Huijing, YU Meng, WANG Fengru, KE Shaoying
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College of Life Sciences,Agricultural University of Hebei,Baoding,Hebei 071033,China
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| Abstract: |
| 【Objective】The study aimed to clone the HDR gene from Atractylodes chinensis (DC.) Koidz. and investigate its biological characteristics and expression patterns in different tissues and under salt stress,providing a theoretical basis for studying the functional role of the AcHDR gene.【Method】The AcHDR gene was cloned using RACE technology.The physicochemical properties,conserved domains,conservation,and structure of the AcHDR-encoded protein were analyzed,and a phylogenetic tree was constructed.The expression of AcHDR in different tissues and under 100 mmol/L NaCl stress at different time points was detected using RT-qPCR technology.The photosynthetic characteristics of leaves under different salt stress levels were also determined.【Result】The full-length open reading frame of AcHDR is 1 383 bp (GenBank accession number:PQ369427),encoding 460 amino acids,and contains a lytB_ispH domain.It is relatively conserved during evolution and closely related to HDR genes from lettuce,chicory,and rubber grass.RT-qPCR results indicated that AcHDR is most highly expressed in the leaves of A.chinensis.Under 100 mmol/L NaCl stress at different time points,the expression of AcHDR initially increased and then decreased.Salt stress at 150 and 250 mmol/L NaCl significantly reduced the photosynthetic characteristics of A.chinensis.【Conclusion】The rapid response of the AcHDR gene to salt stress may enhance the salt tolerance of plants. |
| Key words: Atractylodes chinensis (DC.) Koidz. HDR RT qPCR RACE technology |
