| 摘要: |
| 【目的】对小檗碱引起拟南芥代谢通路的变化进行检测与验证，解析小檗碱的作用机制，为小檗碱作为免疫诱抗剂的研究及应用提供理论依据。【方法】通过转录组测序（RNA-sequencing）技术对小檗碱处理组（XBJ）和对照组（CK）拟南芥样本进行基因表达量测序及分析。通过基因本体（gene ontology，GO）数据库对差异表达基因进行功能注释，并基于京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）进行通路映射。利用RT-qPCR、生理生化测定、非损伤微测技术对差异显著的信号通路相关因子进行验证。【结果】与对照组相比，小檗碱处理12 h后，小檗碱处理组拟南芥共有690个差异表达基因，其中上调表达基因581个，下调表达基因109个。GO富集分析结果表明，小檗碱处理后差异表达基因富集的生物学功能与植物应激反应紧密相关。KEGG富集分析结果表明，小檗碱处理后差异表达基因显著富集于植物免疫激活通路。小檗碱引起拟南芥活性氧和Ca2+信号上调，激活氧化防御系统，经过MAPK级联途径激活免疫转录因子，调控抗病蛋白及其他抗病因子的表达。此外，小檗碱促进了植物次生代谢产物的合成，增强了自身免疫力以应对外源生物或非生物胁迫。【结论】小檗碱诱导拟南芥体内胁迫应激反应，激活了活性氧和Ca2+信号、MAPK级联、抗氧化系统和苯丙烷类合成等多个抗病途径，增强了拟南芥的抗病能力。 |
| 关键词: 小檗碱；诱导抗病性；信号通路；防御反应；抗病因子 拟南芥 |
| DOI：10.13207/j.cnki.jnwafu.2025.12.013 |
| 分类号: |
| 基金项目:宁夏回族自治区重点研发计划项目（2023BCF01053） |
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| Detection and verification of berberine-induced disease resistance signaling pathways in Arabidopsis |
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YANG Xiaocong, BAI Junlong, HONG Bo, WANG Xinpu, SHI Juan, GUO Wenhui
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College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021，China
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| Abstract: |
| 【Objective】The study aimed to investigate and validate the berberine-induced alterations in metabolic pathways in Arabidopsis thaliana,and elucidate the mechanism of its action,thereby providing a theoretical foundation for the research and application of berberine as a plant immunity elicitor.【Method】Transcriptomic profiling via RNA-sequencing was performed on berberine-treated (XBJ) and control (CK) Arabidopsis samples to quantify gene expression.Differentially expressed genes (DEGs) were functionally annotated using the gene ontology (GO) database and mapped to biological pathways via the Kyoto encyclopedia of genes and genomes (KEGG).Key signaling pathways were validated through RT qPCR,physiological and biochemical assays,and non-invasive micro-test technology (NMT).【Result】At 12 h post-treatment,690 DEGs (581 up-regulated,109 down-regulated) were identified in XBJ group compared to CK group.GO enrichment analysis revealed that DEGs were predominantly associated with plant stress responses.The KEGG enrichment results showed significant enrichment of DEGs in the plant immunity activation pathways.Berberine triggered the upregulation of reactive oxygen species (ROS) and Ca2+ signaling,activated oxidative defense systems,and induced immune transcription factors via the MAPK cascade pathway,thereby regulating the expression of disease resistance proteins and other defense related factors.Additionally,berberine enhanced the synthesis of secondary metabolites,bolstering the immunity against biotic and abiotic stresses in Arabidopsis.【Conclusion】Berberine enhances disease resistance in Arabidopsis by inducing stress-responsive pathways,including ROS/Ca2+ signaling,MAPK cascades,antioxidant systems,and phenylpropanoid biosynthesis,collectively priming a multi-layered defense mechanism. |
| Key words: berberine induced resistance signaling pathway defense responses disease resistance factors Arabidopsis |
