| 摘要: |
| 【目的】明确次生代谢物质芦丁对鳞翅目昆虫家蚕取食及生长发育的影响，为开发新型生物防治药剂提供基础，并推进家蚕模式生物系统在鳞翅目害虫防治领域的实际应用。【方法】采用叶碟法，以蒸馏水处理的桑叶为对照，用不同质量浓度(5,10和20 mg/mL)芦丁处理的桑叶对家蚕进行拒食与产卵趋避试验，同时在室内对二龄和三龄家蚕分别饲喂不同质量浓度(5,10和20 mg/mL)芦丁处理的桑叶，喂食一定时间后，测定家蚕存活率、体质量、发育历期，探究芦丁对家蚕生长发育的影响。【结果】黄酮类化合物芦丁对二龄及三龄家蚕幼虫均具有拒食活性，且随着质量浓度的增大，芦丁对家蚕幼虫的拒食活性也随之增强；随着取食时间的延长，芦丁对家蚕幼虫的拒食活性逐渐降低，拒食率一般在刚取食时最高；芦丁对二龄与三龄家蚕幼虫拒食活性的影响差异不大。取食芦丁的家蚕幼虫均能够正常生长发育，但随着芦丁质量浓度的增加，二龄和三龄家蚕幼虫的发育历期逐步延长，表明芦丁对家蚕生长发育有一定的抑制作用，尤以20 mg/mL芦丁处理对家蚕生长发育的抑制作用最强。取食不同质量浓度芦丁处理的桑叶，家蚕产卵趋避率均大于50%，表明芦丁对家蚕成虫有较强的产卵趋避作用。【结论】黄酮类化合物芦丁在高质量浓度下对鳞翅目家蚕有一定的震慑和驱赶作用，对其生长发育有明显抑制作用，具有开发为生物农药的潜力。 |
| 关键词: 芦丁 家蚕 拒食活性 生长发育 生物农药 |
| DOI：10.13207/j.cnki.jnwafu.2025.01.009 |
| 分类号: |
| 基金项目:吉林省科技发展计划项目“基于人参内生菌的生防制剂与示范应用”(20170204018YY）；吉林省科技发展计划项目“我省中东部地区人参林下仿生态栽培技术研究”（20210202084NC) |
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| Effects of rutin on feeding and growth of silkworm |
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XIA Zhongjie, WU Mengjia, LEI Fengjie, ZHANG Aihua
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College of Chinese Medicinal Material，Jilin Agricultural University，Changchun，Jilin 130118，China
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| Abstract: |
| 【Objective】This study determined the effects of secondary metabolite rutin on feeding and growth of Lepidopteran silkworm to provide basis for developing new biological control agents and promoting practical application of silkworm model biological system in the field of Lepidopteran pest control.【Method】Leaf plate method was used to test feeding resistance and oviposition avoidance of mulberry leaves treated with different mass concentrations (5,10,and 20 mg/mL) of rutin.Distilled water was used as control.Mulberry leaves treated with different mass concentrations (5,10,and 20 mg/mL) of rutin were fed to the second and third aged silkworms in laboratory for certain periods of feeding time.Survival rate,body weight and development period of silkworms were measured to explore the effects of rutin on growth and development of silkworms.【Result】Flavonoid rutin had anti-feeding activity on both the second and third instars of silkworm larvae and the activity increased with the increase of mass concentration.With the prolongation of feeding time,the anti-feeding activity of rutin on silkworm larvae gradually decreased with the highest anti-feeding rate at the beginning of feeding.There was no significant difference between the second and third instars.With the increase of rutin concentration,the development period of the second and third instar larvae was gradually extended,indicating that rutin had inhibitory effects on growth period,especially in the 20 mg/mL treatment group.The oviposition evasion rate of all rutin treatments was greater than 50%,indicating that rutin had good oviposition evasion effects on adult silkworm.【Conclusion】Flavonoid rutin had certain deterrent and repelling effects on growth and development of Lepidoptera Bombyx mori at high concentrations,with the potential to be developed as bio-pesticide. |
| Key words: rutin Bombyx mori anti-feeding activity growth and development bio-pesticide |
