摘要: |
【目的】探究牦牛3个部位肌肉品质差异的分子机理。【方法】采集牦牛肩肉(shoulder,Sh)、外脊肉(striploin,St)、黄瓜条(silverside,Si),采用高通量测序方法对其转录组进行测序,数据经过滤后采用FPKM筛选Sh/Si、St/Si、St/Sh的差异表达基因,对差异表达基因进行GO功能富集分析和KEGG通路分析。随机选取4个差异表达基因(2个上调基因BARX2和HOXA13,2个下调基因HOXC8和LOC112441511),采用实时荧光定量PCR法(RT-qPCR)对其表达水平进行验证。【结果】测序后对数据质量进行分析,结果表明测序数据质量可靠。差异表达分析显示,Sh/Si的差异表达基因为607个,上调和下调基因分别为419和188个;St/Si的差异表达基因为713个,上调和下调基因分别为445和268个; St/Sh的差异表达基因为295个,上调和下调基因分别为132和163个。GO功能注释分类与KEGG通路分析发现,3个部位差异表达基因的主功能有离子运输、离子跨膜运输、细胞核、肌动蛋白基础纤维突起和阴离子输运等,富集的主要信号通路有氧化磷酸化、氮代谢和MAPK等。RT-qPCR结果证实了RNA-Seq结果的可靠性。【结论】NADH脱氢酶(泛醌)Fe-S蛋白(NDUFS6)、NADH脱氢酶(泛醌)1 -亚复合物亚基8(NDUFB8)、成纤维细胞成长因子-7(FGF7)、细丝蛋白C(FLNC)、碳酸酐酶 14前体(CA14)和碳酸酐酶3(CA3)基因等可能是牦牛肉品质调控的主要基因。 |
关键词: 牦牛 肌肉品质 转录组测序 信号通路 生物功能 特征基因 |
DOI:DOI:10.13207/j.cnki.jnwafu.2024.12.001 |
分类号: |
基金项目:青海省“高端创新人才千人计划”项目;青海省科技计划项目(2023-NK-135);国家重点研发计划项目(2022YFD-1602304-4);青海省中央引导地方科技发展资金项目(2023ZY027) |
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Molecular mechanism of muscle quality differences in different parts of yak |
LIU Yunna,HU Rong,WU Haiyue,YAN Zhongxin,XIANG Yang,LIN Qing
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1.College of Agriculture and Animal Husbandry,Qinghai University, Xining,Qinghai 810016,China;2.Academy of Animal Husbandry and Veterinary Medicine,Qinghai University, Xining,Qinghai 810016,China
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Abstract: |
【Objective】 This study explored the molecular mechanism behind the differences in muscle quality among three parts of yak.【Method】Muscle samples from shoulder (Sh),striploin (St),and silverside (Si) of yak were collected and subjected to high throughput sequencing to analyze transcriptomes.The data were filtered and differentially expressed genes (DEGs) between Sh/Si,St/Si and St/Sh were selected using FPKM for GO functional clustering analysis and KEGG pathway analysis.Four DEGs,including two upregulated genes (BARX2 and HOXA13) and two downregulated genes (HOXC8 and LOC112441511),were randomly selected for validation of expression levels using real-time quantitative PCR (RT-qPCR).【Result】Post-sequencing data quality analysis confirmed the reliability of sequencing data.Differential expression analysis revealed that there were 607 DEGs between Sh/Si,with 419 upregulated and 188 downregulated.There were 713 DEGs between St/Si,with 445 upregulated and 268 downregulated,and there were 295 DEGs between St/Sh,with 132 upregulated and 163 downregulated.GO annotation and KEGG pathway enrichment analyses showed that main functions of DEGs in the three parts related to ion transport,transmembrane ion transport,nuclear functions,actin filament-based process and anion transport,and major enrichment pathways included oxidative phosphorylation,nitrogen metabolism and MAPK signaling pathway.The RT-qPCR results confirmed the reliability of RNA-Seq data.【Conclusion】NADH dehydrogenase (ubiquinone) Fe-S protein (NDUFS6),NADH dehydrogenase (ubiquinone) 1-subcomplex subunit 8 (NDUFB8),fibroblast growth factor-7 (FGF7),thin filament protein C (FLNC),carbonic anhydrase 14 precursor (CA14) and carbonic anhydrase 3 (CA3) may be characteristic genes for regulation of yak meat quality. |
Key words: yak muscle quality RNA-Seq signaling pathway biological function signature gene |