| 摘要: |
| 【目的】探讨云南栘［木衣］种子萌发至成苗期间8种内源激素的变化规律及其相互作用，为云南栘［木衣］产业化发展提供理论依据。【方法】以云南省澜沧县糯扎渡镇的云南栘［木衣］种子为材料，根据前期种子吸水量确定种子的干种期(S1)和吸胀期(S2)；再根据不同阶段种子的外部形态特征变化，确定种子的萌动期(S3)、出苗期(S4)、成苗期(S5)，分析种子含水量、发芽率和吸水特征。采用超高液相色谱串联质谱（UPLC-MS/MS）测定5个时期内种子的脱落酸(ABA)、吲哚乙酸(IAA)、玉米素核苷(tZR)、赤霉素(GA3)、茉莉酸(JA)、水杨酸(SA)、乙烯(ETH)、独脚金内酯(SL)含量，分析其动态变化及激素含量的比值，并对5个时期8种内源激素含量进行相关性分析和主成分分析。【结果】50粒云南栘［木衣］种子平均含水量为10.49%,发芽率为96.00%，根据种子吸水特征及外部形态特征变化确定0，3，11，18，27 d为种子萌发至成苗期间的5个时期。ABA、JA含量在S1-S3时期大量降低，IAA、tZR、GA3、SA、ETH含量在S2-S4时期均有增长趋势，GA3、ETH含量在S4-S5时期持续增加。IAA、tZR、SA、ETH与ABA、JA含量的比值在S3时期显著增大；GA3、SL与ABA、JA含量的比值在S4时期显著增大。8种内源激素含量在5个时期(S1-S5)差异较大。ABA、JA与IAA、tZR、GA3、SL含量存在极显著正相关，ABA与JA含量也呈极显著正相关，IAA、tZR、GA3、SL含量间均呈正相关，且与SA、ETH含量呈负相关。【结论】云南栘［木衣］种子在萌发至成苗期间的变化是8种激素共同作用的结果。IAA、tZR、SA、ETH、SL、GA3协同作用能够促进种子萌发及出苗，ABA、JA协同抑制种子萌发。IAA、GA3、tZR、SA、ETH、SL与ABA、JA的比值变化反映了该期间激素平衡关系。 |
| 关键词: 云南栘［木衣］；种子萌发；内源激素；林木育种 激素平衡 |
| DOI：DOI:10.13207/j.cnki.jnwafu.2024.11.004 |
| 分类号: |
| 基金项目:国家自然科学基金项目（32060350）；云南省“万人计划”青年拔尖人才专项（YNWR-QNBJ-2020-230） |
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| Dynamics of endogenous hormones during seed germination to seedling establishment in Docynia delavayi （Franch.） |
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LI Jingting1, CHEN Can1, XU Liu1, MA Haitao1, CHEN Yue1, LI Enliang2, WANG Dawei1
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1.Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in the Southwest China/Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province,College of Forestry,Southwest Forestry University,Kunming,Yunnan 650224,China;2.Yunnan Province Mojiang Xiqisuoluo Provincial Nature Reserve Management Bureau,Puer,Yunnan 654800,China
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| Abstract: |
| 【Objective】This study investigated the change patterns and interactions of eight endogenous hormones from seed germination to seedling establishment in Docynia delavayi to provide basis for the further industrialisation of Docynia delavayi.【Method】Docynia delavayi seeds from Nuozhadu Town,Lancang County,Yunnan Province were used as materials,seed drying stage (S1) and swelling stage (S2) of Docynia delavayi seeds from Nuozhadu Town,Lancang County,Yunnan Province were determined based on the amount of water absorbed by seeds.Changes in external morphological characteristics of seeds at different stages were used to determine seed germination stage (S3),seedling emergence stage (S4) and seedling establishment stage (S5),and the water content,germination rate,and water absorption characteristics of the seeds were analyzed.Contents of eight endogenous hormones including abscisic acid (ABA),indoleacetic acid (IAA),zeatin riboside (tZR),gibberellin (GA3),jasmonic acid (JA),salicylic acid (SA),ethylene (ETH) and strigolactone (SL) were determined by UPLC-MS/MS, their dynamic changes and ratios were analyzed,and the correlation analysis and principal component analysis were conducted for the five periods.【Result】The average moisture content of Docynia delavayi fifty seeds was 10.49%,and the germination rate was 96.00%,and five periods of 0,3,11,18,and 27 d were identified between seed germination and seedling establishment based on changes in seed water absorption characteristics and external morphological features.Contents of ABA and JA decreased considerably from S1 to S3,contents of IAA,tZR,GA3,SA and ETH tended to increase from S2 to S4,and contents of GA3 and ETH continued to increase from S4 to S5.Ratios of IAA,tZR,SA and ETH to ABA and JA increased significantly during S3,while ratios of GA3 and SL to ABA and JA increased significantly during S4.Contents of the eight endogenous hormones varied greatly among all five periods (S1-S5).There were highly significant positive correlations between ABA and JA with IAA,tZR,GA3 and SL contents,and highly significant positive correlations between ABA and JA contents, positive correlations between IAA,tZR,GA3 and SL contents,and negative correlations with SA and ETH contents.【Conclusion】The changes of Docynia delavayi seeds from germination to seedling establishment were resulted from the joint action of eight hormones.IAA,tZR,SA,ETH,SL and GA3 synergistically promoted seed germination and seedling establishment,and ABA and JA synergistically inhibited seed germination.Changes in ratios of IAA,GA3,tZR,SA,ETH and SL to ABA and JA reflected hormone homeostasis during the periods. |
| Key words: Docynia delavayi seed germination endogenous hormone forest tree breeding hormonal balance |
