| 摘要: |
| 【目的】研究鬼针草水提物（BPE）的体外抗伪狂犬病毒（Pseudorabies virus，PrV）作用。【方法】制备640 mg/mL（以生药计）的BPE，检测其对仓鼠肾上皮细胞(BHK-21)的毒性（半数中毒浓度（TC50））及对PrV体外抗性（半数抑制浓度（EC50））和治疗指数（TI）。采用CCK-8法检测BPE抗PrV的机制。采用间接免疫荧光法检测BPE对gB蛋白表达和PrV所致细胞凋亡的影响，采用实时荧光定量PCR方法检测BPE对PrV gB基因表达的影响。【结果】BPE对BHK-21细胞的TC50为28.7 mg/mL，对PrV的EC50为5.16 mg/mL，TI为5.56。BPE抗PrV的机制为抑制PrV增殖和灭活PrV。BPE可抑制PrV诱导的BHK 21细胞凋亡，抑制PrV gB基因和蛋白的表达。【结论】BPE具有明显的体外抗PrV活性。 |
| 关键词: 鬼针草水提物 伪狂犬病毒 BHK-21细胞 |
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| 基金项目:中央引导地方科技发展专项（2019L3011）；龙岩市奇迈基金项目（2018LYQM0201）；预防兽医学与生物技术福建省高等学校重点实验室开放课题（2019KF01） |
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| Effect of aqueous extract of Bidens pilosa L. on Pseudorabies virus |
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GUO Zhijun,XIE Huifan,WU Qiwen,et al
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| Abstract: |
| 【Objective】This study aimed to investigate the protective effect of aqueous extract of Bidens pilosa L.(BPE) on Pseudorabies virus (PrV) in vitro.【Method】The cytotoxicity and anti-PrV activity of BPE at concentration of 640 mg/mL on BHK-21 cells were detected by CCK-8,and the anti PrV mechanism of BPE was investigated by CCK-8.The TC50,EC50 and TI were determined.The indirect immunofluorescence was used to detect the effect of BPE on PrV induced apoptosis and expression of PrV gB protein,and the real time fluorescence quantitative PCR was used to detect the expression of PrV gB gene.【Result】The TC50 of BPE in BHK-21 cells was 28.7 mg/mL,the IC50 of PrV was 5.16 mg/mL,and the TI was 5.56.The mechanism of anti PrV was based on inhibiting PrV proliferation and inactivating PrV.BPE might protect PrV infected BHK-21 cells through suppressing expression of PrV gB gene and protein (P<0.01) and inhibiting PrV-induced apoptosis of BHK-21 cells.【Conclusion】BPE had significant anti-PrV activity in vitro. |
| Key words: aqueous extract of Bidens pilosa L. PrV BHK-21 cells |
