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磷肥对冷冻胁迫下紫花苜蓿根颈抗氧化特性的影响
陈卫东1, 张玉霞1, 丛百明,等2
1.内蒙古民族大学 农学院;2.内蒙古通辽市畜牧兽医科学研究所
摘要:
【目的】探究科尔沁沙地紫花苜蓿抗寒性对磷肥施用种类和用量的响应,为科尔沁沙地苜蓿抗寒越冬提供理论依据和技术指导。【方法】以‘公农1号’ 紫花苜蓿为材料,采用二因素(磷肥种类和施肥量)随机区组试验设计,于秋季施用0,100,200,300,400 kg/hm2(以P2O5计)重过磷酸钙和磷酸二铵,对紫花苜蓿越冬材料人工模拟低温冷冻条件(-20 ℃),以冷藏(4 ℃)为对照,研究紫花苜蓿根颈的丙二醛(MDA)含量、抗氧化酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT))活性和可溶性蛋白(SP)含量的变化,并对上述指标的相关性进行分析。【结果】在低温冷藏和低温冷冻条件下,随着2种磷肥施用量的增加,紫花苜蓿根颈MDA含量呈先降后升的趋势,均在300 kg/hm2(P2O5)处理下最低;紫花苜蓿根颈SOD、POD、CAT活性和SP含量则呈先升后降的趋势,在300 kg/hm2(P2O5)处理下3种酶活性和SP含量达到高点。在施肥量相同时,与施用磷酸二铵相比,施用重过磷酸钙的紫花苜蓿根颈MDA含量更低,SOD、POD、CAT活性和SP含量大多更高。与低温冷藏条件相比,相同肥料处理在低温冷冻条件下紫花苜蓿MDA、SP含量增加,SOD、POD、CAT活性增强,其中在施肥量为300 kg/hm2(P2O5)时,施用重过磷酸钙紫花苜蓿根颈的SP含量最高,SOD、POD、CAT活性最强,分别为38.85 mg/g、135.59 U/g、387.57 U/(min·g)和47.71 U/(min·g)。相关性分析结果表明,在低温冷冻条件下,施用重过磷酸钙和磷酸二铵紫花苜蓿根颈的MDA含量,均与SOD、POD、CAT活性和SP含量呈显著(P<0.05)或极显著负相关(P<0.01)。【结论】2种磷肥均通过提高低温冷冻胁迫下苜蓿根颈的SOD、POD、CAT活性和SP含量,降低MDA含量,提高苜蓿抗寒性。在科尔沁沙地秋季施用300 kg/hm2重过磷酸钙有利于苜蓿安全越冬。
关键词:  紫花苜蓿  磷肥  冷冻胁迫  抗氧化活性
DOI:
分类号:
基金项目:内蒙古自然科学研究基金项目(2020MS03081);国家自然科学基金项目(31960352);内蒙古自治区“双一流”建设一带一路作物学项目(NMDGJ0018)
Physiological and antioxidant activities of phosphate fertilizers on alfalfa roots under freezing stress
CHEN Weidong,ZHANG Yuxia,CONG Baiming,et al
Abstract:
【Objective】This study explored the response of alfalfa to cold resistance in Horqin sandy land to type and dosage of phosphate fertilizers to provide theoretical basis and technical guidance for cold tolerant alfalfa overwintering.【Method】Alfalfa variety of ‘Gongnong No. 1’ was treated using a two-factor (phosphorous fertilizer type and fertilizer amount) randomized block experiment design with 0,100,200,300 and 400 kg/hm2 calcium phosphate and diammonium phosphate (calculated as P2O5) in autumn.Then alfalfa overwintering materials were stored under artificially simulated low-temperature freezing conditions (-20 ℃) using cold storage (4 ℃) as control.Changes in alondialdehyde (MDA) content,activities of antioxidant enzymes (superoxide dismutase (SOD),peroxidase (POD),catalase (CAT)) and soluble protein (SP) content were determined and correlations among above indicators were analyzed.【Result】Under low temperature storage and low-temperature freezing conditions,with the increase of application rates of phosphate fertilizers,MDA contents in root neck of alfalfa decreased first and then increased with the lowest in treatment of 300 kg/hm2 (P2O5).Activities of SOD,POD and CAT and content of SP in root neck showed a trend of increasing first and then decreasing.They all reached peaks in treatment of 300 kg/hm2 (P2O5).With same fertilizer amount,applying double superphosphate led to lower MDA content and higher activities of SOD,POD and CAT and content of SP in comparison of diammonium phosphate.Compared with low-temperature storage conditions,low-temperature freezing conditions caused higher contents of MDA and SP and activities of SOD,POD and CAT at same applying amount.The peak values of 38.85 mg/g,135.59 U/g,387.57 U/(min·g) and 47.71 U/(min·g) were obtained in application of double superphosphate at the application rate of 300 kg/hm2 (P2O5).Under low temperature freezing conditions,MDA contents in root neck of alfalfa applied with double superphosphate and diammonium phosphate had significant (P<0.05) or extremely significant (P<0.01) and negative correlation with activities of SOD,POD and CAT and content of SP.【Conclusion】Both phosphate fertilizers can increase activities of SOD,POD and CAT as well as SP content in root neck of alfalfa under low temperature freezing stress,reduce MDA content,and improve cold resistance.Application of 300 kg/hm2 double superphosphate in autumn is beneficial to safe overwintering of alfalfa in Horqin sandy land.
Key words:  alfalfa  phosphate fertilizer  freezing stress  antioxidant properties