引用本文:
【打印本页】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 170次   下载 140 本文二维码信息
码上扫一扫!
分享到: 微信 更多
尖裸鲤染色体核型分析及进化地位研究
马 凯1,2, 佟广香1,2, 张永泉,等1,2
1.中国水产科学研究院 黑龙江水产研究所;2.黑龙江省冷水性鱼类种质资源及增养殖重点开放实验室
摘要:
【目的】对尖裸鲤(Oxygymnocypris stewartii)的染色体核型进行分析,为其进化生物学、种质标准制定及杂交育种亲本选择提供细胞遗传学依据。【方法】以西藏自治区农牧科学院水产科学研究所和中国水产科学研究院黑龙江水产研究所联合驯养的尖裸鲤为试验对象,取其头肾采用冷滴片法制备染色体标本,PI染色,荧光显微镜下拍照,使用Photoshop和曲线测量工具E ruler对染色体进行测量和配对,获得染色体核型,并与已报道的19种(或亚种)裂腹鱼亚科鱼类的中部着丝粒染色体(m)+亚中部着丝粒染色体(sm)和亚端部着丝粒染色体(st)+端部着丝粒染色体(t)的数量比进行比对,分析尖裸鲤的进化地位。【结果】尖裸鲤染色体数目为2n=92,核型公式为26m+28sm+20st+18t,臂数(NF)=146,进化地位在裂腹鱼亚科中处于中等水平。【结论】明确了尖裸鲤的染色体核型和进化地位,其特化程度为高度特化等级。
关键词:  尖裸鲤  染色体核型  裂腹鱼亚科  进化地位
DOI:
分类号:
基金项目:农业财政专项“西藏重点水域渔业资源与环境调查”;中国水产科学研究院基本科研业务费项目(2020TD56)
Karyotype analysis and evolutionary status of Oxygymnocypris stewartii
MA Kai,TONG Guangxiang,ZHANG Yongquan,et al
Abstract:
【Objective】The karyotype of Oxygymnocypris stewartii was analyzed to provide cytogenetic basis for its development of evolutionary biology,germplasm standards and parental selection in cross breeding.【Method】The head kidney of O. stewartii,domesticated by Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences and Heilongjiang River Fishery Research Institute of Chinese Academy of Fishery Sciences,was collected and cold drop method was used to prepare chromosome specimens.After PI staining and fluorescence microscopy,the software of Photoshop and curve measurement tool E-ruler were used to measure and pair chromosomes of O. stewartii,and the evolutionary status of O. stewartii was analyzed by comparing the chromosome ratios of metacentric chromosome (m)+submetacentric chromosome (sm) and subtermocentric chromosome (st)+telocentric chromosome (t) with 19 species (subspecies) of Schizothoracinae.【Result】The chromosome number of O. stewartii was 2n=92,the karyotype formula was 26m+28sm+20st+18t,the arm number (NF)=146,and its evolutionary status in Schizothoracinae was middle level.【Conclusion】The karyotype of O. stewartii and its evolutionary status were determined,and the degree of specialization was high.
Key words:  Oxygymnocypris stewartii  karyotype  Schizothoracinae  evolutionary status