| 摘要: |
| 【目的】探明安徽马鞍山地区白山羊品种内的群体遗传多样性, 为马鞍山白山羊品种资源保护、开发和遗传育种提供参考依据。【方法】从安徽马鞍山地区分别采集品种特征明显、无血缘关系的公羊、母羊个体血样19和40份，提取血液基因组DNA，通过PCR扩增公羊SRY基因、 母羊线粒体DNA D-loop区序列并测序，并利用DNAman、DNAsp和MEGA X软件对其进行遗传进化分析。【结果】马鞍山地区白山羊公羊SRY基因编码区共含有92个变异位点，占核苷酸总数的10.50%，共含有19种单倍型，单倍型多样性（Hd）为1.000，核苷酸多样性（Pi）为0.018 68，平均核苷酸差异数（k）为15.152；母羊mtDNA D-loop区共含有986个变异位点，占核苷酸总数的69.24%，共含有32种单倍型，单倍型多样性（Hd）为0.986，核苷酸多样性（Pi）为0.127 13，平均核苷酸差异数（k）为12.915。构建马鞍山地区白山羊与国内其他20个山羊品种间的系统发育树，结果显示,马鞍山地区白山羊首先与黄淮山羊、西藏山羊聚为一支；其次与辽宁绒山羊聚为一支；再与贵州白山羊聚为一支；最后与安哥拉山羊、波尔山羊聚为一支。【结论】马鞍山地区白山羊拥有丰富的遗传多样性，在起源中受到较多的其他羊种群体扩张的影响。 |
| 关键词: 白山羊 线粒体DNA D-loop区 SRY基因 遗传多样性 马鞍山地区 |
| DOI： |
| 分类号: |
| 基金项目:安徽省牛羊产业技术体系项目（AHCYJSTX-07） |
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| Genetic evolution analysis on mitochondrial DNA D-loop region and SRY gene of Maanshan white goat |
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YANG Xinchun,ZHU Yixiao,LIU Hongyu,et al
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| Abstract: |
| 【Objective】This study explored the genetic diversity of Maanshan white goat to provide reference for its protection,development and genetic breeding.【Method】A total of 19 rams and 40 ewes unrelated individuals with significant breeding characteristics were selected in Maanshan.Genomic DNA was extracted from blood,and SRY gene of ram and mtDNA D-loop region of ewes were amplified and sequenced using PCR.The sequences were used to analyze genetic evolution by DNAman,DNAsp and MEGA X.【Result】A total of 92 variable sites were obtained in coding region of SRY gene in ram of Maanshan white goat,which accounted for 10.50% of total nucleotides including 19 haplotypes.The haplotype diversity (Hd) was 1.000,nucleotide diversity (Pi) was 0.018 68,and average nucleotide difference (k) was 15.152.A total of 986 variable sites were obtained in mtDNA D-loop region of ewes.They accounted for 69.24% of total nucleotides including 32 haplotypes with haplotype diversity (Hd) of 0.986,nucleotide diversity (Pi) of 0.127 13 and mean nucleotide difference number (k) of 12.915.The phylogenetic tree between Maanshan white goat and 20 other goat breeds in China showed that Maanshan white goat firstly clustered with Huanghuai goat and Tibetan goat.They also grouped with Liaoning cashmere goat,Guizhou white goat,as well as Angora goat and Boer goat sequentially.【Conclusion】Maanshan white goats had abundant genetic diversity and were affected by other breeds in evolution. |
| Key words: white goat mtDNA D-loop region SRY gene genetic diversity Maanshan region |
