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温度和干旱胁迫下绳状青霉菌P1的溶磷能力研究
张建峰1, 王冠雄1, 黄 佳,等1
吉林农业大学 生命科学学院
摘要:
【目的】探究绳状青霉菌(Penicillium funicuiosum)P1对绿豆种子萌发的作用及其在温度和干旱胁迫下的溶磷能力,为生物磷肥的研制提供菌种资源和理论依据。【方法】将绿豆种子消毒后,用沾蘸法沾绳状青霉菌P1菌悬液,置于铺有2层湿滤纸的无菌培养皿中28 ℃培养4 d,以沾无菌水的处理为对照,测定绿豆种子的发芽率、发芽势、鲜质量、干质量、芽长、根长、发芽指数和活力指数,分析P1对绿豆种子萌发的影响。测定不同干旱程度(极轻度、轻度、中度、重度)和不同温度(5,10,12,15,20,25,28,35和40 ℃)条件下P1的溶磷能力,分析环境胁迫对P1解磷能力的影响。【结果】绳状青霉P1对绿豆种子萌发具有良好的促生效果,与对照相比,绳状青霉P1使绿豆幼苗的芽长和根长分别增长了149.14%和50.21%,发芽率提高了86.67%,种子活力提升了237.25%。绳状青霉P1在干旱胁迫下仍具有很强的溶磷能力,在重度干旱胁迫下的溶磷量可达418.85 mg/L。P1在10~35 ℃的条件下均能生长且具备溶磷能力,10 ℃条件下的溶磷量为34.67 mg/L,28 ℃条件下的溶磷量为1 120.52 mg/L,35 ℃条件下的溶磷量为364.97 mg/L。【结论】绳状青霉菌P1是一株对环境适应性较好且能促进植物生长的菌株,可用于制备微生物菌肥。
关键词:  溶磷真菌  溶磷能力  绳状青霉菌  干旱胁迫  温度胁迫
DOI:
分类号:
基金项目:国家自然科学基金国际合作与交流项目(41920104008);吉林省教育厅项目(JJKH20180687KJ);吉林省科技厅项目(20190303070SF);长春市科技局项目(18DY020)
Effects of Penicillium funiculosum P1 on P-solubilization ability under temperature and drought stress
ZHANG Jianfeng,WANG Guanxiong,HUANG Jia,et al
Abstract:
【Objective】The effects of Penicillium funiculosum P1 on germination of mung bean seeds and its P-solubilization ability under temperature and drought stress were studied to provide microorganism resources and basis for development of biophosphate fertilizer.【Method】The sterilized mung beans were dipped with P1 bacterial suspension and placed in a sterile Petri dish with two layers of wet filter paper and cultured at 28 ℃ for 4 days.Taked sterile water treatment as control.The germination rate,germination potential,fresh weight,dry weight,bud length,root length,germination index and seed vigor of mung beans were determined to analyze the effect of P1 on germination of mung beans.The effect of environmental stress on P-solubilization ability of P1 was also analyzed by studying the P-solubilization ability of P1 under different drought degree (extremely mild drought,mild drought,moderate drought,severe drought) and temperature (5,10,12,15,20,25,28,35,40 ℃)stresses.【Result】Penicillium funiculosum P1 had good effects on germination of mung beans,and shoot length,root length,germination rate and seed vigor were increased by 149.14%,50.21%,86.67% and 237.25%,respectively.P1 still had strong P-solubilization ability under drought stress,and the amount of dissolved phosphorus reached 418.85 mg/L.P1 could grow at 10-35 ℃ with p-solubilization ability.The amount of dissolved phosphorus reached 34.67 mg/L at 10 ℃, 1 120.52 mg/L at 28 ℃,and 364.97 mg/L at 35 ℃.【Conclusion】With good environmental adaptability and ability of promoting plant growth,Penicillium funicuiosum P1 has the potential to prepare biophosphate fertilizer.
Key words:  phosphate solubilizing fungi  P-solubilization ability  Penicillium funicuiosum  drought stress  temperature stress