| 摘要: |
| 【目的】建立脱毒、高效的分蘖洋葱微鳞茎盘快繁技术体系，为分蘖洋葱种苗快繁技术体系的建立提供参考。【方法】以分蘖洋葱茎尖为外植体，研究不同质量浓度的激素组合（NAA、6-BA、ZT和GA-3）对分蘖洋葱茎尖启动的影响；探讨不同质量浓度的6-BA（0.1，0.4，1.0 mg/L）与NAA（0.1，1.0，2.0 mg/L）组合和6-BA（0.1，0.4，1.0 mg/L）与IAA（0.1，1.0，2.0 mg/L）组合对分蘖洋葱微鳞茎盘增殖效果的影响；研究MS、1/2 MS和1/4 MS加入0.1 mg/L NAA的生根培养基对分蘖洋葱组培苗生根的影响，并对生根的分蘖洋葱组培苗进行移栽驯化。【结果】分蘖洋葱最佳茎尖启动培养基为MS+0.1 mg/L NAA，成苗率达87.50%；微鳞茎盘最佳增殖培养基为MS+0.4 mg/L 6-BA+0.1 mg/L NAA，平均增殖系数达16.05；最佳生根培养基为1/2 MS+0.1 mg/L NAA，生根率达100%；将生根后的试管苗移栽于V（草炭）∶V（田土）=2∶1的基质中，覆膜保湿，在20 ℃、相对湿度80%条件下培养20 d，移栽驯化成活率可达95%以上。【结论】初步建立了分蘖洋葱微鳞茎盘快繁技术体系，在该体系下分蘖洋葱茎尖成苗率、微鳞茎盘增殖系数、生根率及移栽驯化后的组培苗成活率均较高。 |
| 关键词: 分蘖洋葱 组织培养 微鳞茎盘 快繁技术体系 |
| DOI： |
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| 基金项目:吉林省现代农业产业技术体系建设专项（2013026） |
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| Establishment of rapid propagation system for tiller onion |
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SU Xuejiao,WANG Xiufeng,ZHANG Yue,et al
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| Abstract: |
| 【Objective】This study established a highly effective and virus-free system for rapid propagation of tiller onion microscale stem disc to provide basis for the establishment of a rapid propagation technology system for tiller onion seedlings.【Method】The effects of different concentrations of hormones (NAA,6-BA,ZT and GA-3) on initiation of tiller onion stem tips were studied.The effects of combination of 6-BA (0.1,0.4 and 1.0 mg/L) with NAA (0.1,1.0 and 2.0 mg/L) and 6-BA (0.1,0.4 and 1.0 mg/L) with IAA (0.1,1.0 and 2.0 mg/L) on the proliferation of microscale stem disc of tiller onion were analyzed.The effects of MS,1/2 MS and 1/4 MS plus 0.1 mg/L NAA rooting medium on rooting of tiller onion tissue culture seedlings were also studied and rooting tissue cultured seedlings were transplanted and acclimatized.【Result】The optimum stem tip starting medium of tiller onion was MS+0.1 mg/L NAA with seedling formation rate of 87.50%. The optimum medium for proliferation of microscale stem disc was MS+0.4 mg/L 6-BA+0.1 mg/L NAA with an average proliferation coefficient of 16.05.The optimum rooting medium was 1/2 MS+0.1 mg /L NAA with rooting rate of 100%.The test tube seedlings after rooting were transplanted into a matrix with a ratio of V (peat)∶V (field soil)=2∶1.After film mulching and moisturizing,under the condition of 20 ℃ and 80% relative humidity,the survival rate of transplanting domestication was larger than 95%.【Conclusion】The rapid propagation technology system of tiller onion microscale stem disc was established,which obtained high seedling formation rate,microscale disc proliferation coefficient,rooting rate and survival rate of tissue cultured seedlings. |
| Key words: tiller onion tissue culture microscale disc rapid propagation technology system |
