| 摘要: |
| 【目的】探讨5种藏药、7味中药对藏猪源沙门氏菌的抑菌效果。【方法】采用煎煮法制备5种藏药（石榴健胃丸、仁青芒觉、十五味黑药丸、仁青常觉、仁青二十五味马宝丸等藏药方剂）、7味中药（石榴皮、诃子、炒白术、黄连、败酱草、红藤、薏苡仁）的水煎剂，再采用打孔法制备藏药及中药药敏片。采用药敏纸片法检测33株西藏藏猪源沙门氏菌对5种藏药、7味中药及6种常用抗菌药物的敏感性，并对黄连、仁青芒觉进行体内抑菌试验。【结果】33株藏猪源沙门氏菌对藏药仁青芒觉普遍表现为敏感（69.70%），对中药黄连普遍表现为敏感（69.70%），对氯霉素（100%）、链霉素（100%）、丁胺卡那（100%）、庆大霉素（100%）等4种常用抗菌药物均表现为敏感。体内抑菌试验表明：灌胃营养肉汤的对照组小鼠大多数死亡，致死率达到95.45%，说明攻毒液对小鼠均具有高致病性；仁青芒觉或中药黄连组小鼠死亡率明显降低，保护率均达90%以上。【结论】仁青芒觉和黄连对藏猪源沙门氏菌具有一定的抑制作用，氯霉素、链霉素、丁胺卡那、庆大霉素对藏猪源沙门氏菌敏感，且抗菌药物对藏猪源沙门氏菌的抑菌效果比仁青芒觉和黄连好，在抗生素耐药性泛滥的形势下，仁青芒觉和黄连是防治沙门氏菌病一个很不错的选择。 |
| 关键词: 藏猪 藏药 中药 沙门氏菌 抑菌活性 |
| DOI： |
| 分类号: |
| 基金项目:江苏省人兽共患病学重点实验室资助项目（R1801） |
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| Antibacterial activity of five Tibetan medicine and seven Chinese traditional medicines on Salmonella from Tibetan pigs |
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WANG Gang,ZHAO Yanjuan,JIAO Xin’an,et al
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| Abstract: |
| 【Objective】The antibacterial effects of 5 Tibetan medicines,7 Chinese traditional medicines against Salmonella from Tibetan pigs were studied.【Method】Decoction of the 5 Tibetan medicine (Pomegranate Jianwei pill,Renqingmangjue,Fifteen flavored black pills,Renqingchangjue,and Renqing twenty-five flavored mabao pills) and the 7 Chinese traditional medicines (Pomegranate peel,Myrobalan,Coptis chinensis,Rhizoma atractylodis macrocephalae,Herba patriniae,Sargentgloryvine stem,and Coix seed) was prepared separately.Then,the punching method was used to prepare tablets.The K-B method was used to determine susceptibility against 33 Salmonella from Tibetan pigs.In addition,Coptis chinensis and Renqingmanjue were used for antibacterial test in vivo.【Result】The 33 Salmonella from Tibetan pigs were generally sensitive to Renqingmangjue (69.70%),Coptis chinensis (69.70%) and 4 antibacterial drugs of chloramphenicol (100%),streptomycin (100%),amikacin (100%) and gentamicin (100%).Antibacterial test in vivo showed that the group with nutritious broth had the mortality rate of 95.45%,indicating that the venom was highly pathogenic to mice.The death situation was significantly reduced by Renqingmangju and Coptis chinensis,with protection rate of over 90%.【Conclusion】Renqingmangjue and Coptis chinensis could be used to cure Salmonella from Tibetan pigs.Chloramphenicol,streptomycin,amikacin,tetracycline and gentamicin were sensitive to Salmonella from Tibetan pigs and the antibacterial effects were better than tested Tibetan medicines and Chinese traditional medicines.Renqingmangjue and Coptis chinensis were good to prevent Salmonella in the situation of affluent drug resistance. |
| Key words: Tibetan pigs Tibetan medicine Chinese medicine Salmonella antibacterial activity |
