| 摘要: |
| 【目的】探究蛋鸡育成期和产蛋高峰期相关生殖激素含量及其受体基因mRNA表达量在24 h内的变化规律与差异，分析血液生殖激素含量变化与产蛋排卵行为的关系。【方法】以育成期和产蛋高峰期的罗曼蛋鸡为研究对象，监测其在产蛋高峰期的产蛋时间点，运用酶联免疫吸附法（ELISA）测定2个时期蛋鸡血液中前列腺素（PGs）、雌二醇（E2）、促卵泡素（FSH）、黄体生成素（LH）含量在24 h内的动态变化，实时荧光定量PCR技术检测相应时间点的前列腺素F受体（PTGFR）、雌激素受体（ERα）、促卵泡素受体（FSHR）和黄体生成素受体（LHR）基因mRNA在输卵管子宫部的日表达水平。【结果】蛋鸡产蛋时间集中在早上08:00-10:00。产蛋高峰期时，一天中06:00、10:00、14:00、18:00的PGs含量显著高于育成期（P<0.05），E2含量在06:00和18:00极显著高于育成期（P<0.01），FSH含量在18:00显著高于育成期（P<0.05），LH含量在14:00、22:00和02:00显著高于育成期（P<0.05）。育成期除LH外，其余3种激素含量在一天中波动不大。产蛋高峰期PGs含量在产蛋前显著高于产蛋后，PTGFR mRNA表达量在产蛋前后4 h均显著（P<0.05）升高；FSH和E2含量在产蛋后（18:00）极显著（P<0.01）上升。子宫组织的激素受体基因表达量变化与相应血液激素含量变化一致。【结论】蛋鸡产蛋时间集中于上午，产蛋高峰期各生殖激素含量总体高于育成期，蛋鸡育成期和产蛋高峰期的血液生殖激素水平变化规律与输卵管子宫部激素受体基因mRNA表达量的变化规律基本一致。PGs参与蛋鸡产蛋高峰期的产蛋和排卵过程，E2和FSH可能在蛋壳形成过程中发挥调控作用。 |
| 关键词: 蛋鸡 生殖激素 前列腺素（PGs） 激素受体 前列腺素F受体（PTGFR） |
| DOI： |
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| 基金项目:现代农业产业技术体系项目（CARS-41）；国家自然科学基金面上项目（31872347）； 四川省科技计划项目（2018NZZ-J003） |
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| Reproductive hormone levels and expression change of receptor genes in growing and laying periods of hens |
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LI Fugui,LIU Jia,MIAO Xiaomeng,et al
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| Abstract: |
| 【Objective】This study investigated the changes and differences of reproductive hormones and the expression of receptor genes 24 hours within growing and laying periods of laying hens,and analyzed the relationship between changes of hormone contents and ovulation oviposition behaviors.【Method】Rohman laying hens during growth and egg-laying peak periods were selected and laying points were monitored.Daily changes of prostaglandins (PGs),estrogens (E2),follicle stimulating hormones (FSH) and luteinizing hormone (LH) in blood were determined via ELISA.The mRNA expression of genes prostaglandin F receptor (PTGFR),estrogen receptor (ERα),follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in uterus of oviduct were detected by real-time fluorescence quantitative PCR.【Result】The oviposition behaviors mainly happened between 08:00 and 10:00 in the morning.PGs in laying period was significantly higher than that in growing period at 06:00,10:00,14:00 and 18:00 (P<0.05).E2 in laying period was very significantly higher than that in growing period at 06:00 and 18:00 (P<0.01).FSH was significantly higher than that in growing period at 18:00 (P<0.05) and LH at 14:00,22:00 and 02:00 in laying period was significantly higher than that in growing period (P<0.05).The diurnal variation of other three hormones except LH was slight in the growing period.The content of PGs in the peak period of laying eggs was significantly higher than that before laying eggs.The mRNA expression of PTGFR increased significantly 4 hours before and after laying eggs (P<0.05).FSH and E2 significantly increased after laying eggs at 18:00 (P<0.01).The changes in expression of hormone receptor genes in uterus tissue were consistent with the changes of corresponding hormone in blood.【Conclusion】Egg laying mainly happened in the morning,and the contents of reproductive hormones in laying period were higher than those in growing period.The changes of blood reproductive hormone levels and uterine hormone receptor genes,expression in oviduct were consistent.PGs involved in laying and ovulation process during egg-laying peak period while E2 and FSH may play a regulatory role in eggshell formation. |
| Key words: layer reproductive hormone prostaglandins (PGs) hormone receptor prostaglandin F receptor (PTGFR) |
