| 摘要: |
| 【目的】研究质体囊泡诱导蛋白(VIPP1)运动的调控过程，解析其修复叶绿体被膜的分子机制。【方法】从拟南芥VIPP1-GFP/Col转基因株系中分离叶绿体，在荧光显微镜下观察GTP和Ca2+作用下VPP1-GFP蛋白复合体的运动规律，分析其对该蛋白运动的作用方式，并探讨其作用机制。【结果】离体叶绿体在低渗胁迫下膨胀变大，被膜与类囊体间隙变大并鼓起，VPP1 GFP蛋白复合体开始自由移动。CaCl2可以完全抑制VPP1-GFP蛋白的运动，这种抑制作用由Ca2+而不是Cl-引起。GTP可以有效逆转Ca2+的抑制作用，并再次激活该蛋白的运动。低渗胁迫下，叶绿体被膜完整性的维持依赖于VIPP1蛋白的运动。【结论】拟南芥叶绿体VIPP1蛋白的运动受GTP和Ca2+共同调控，GTP对VIPP1蛋白运动起促进作用，Ca2+对该过程起抑制作用。 |
| 关键词: 叶绿体被膜 VIPP1运动 叶绿体修复 |
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| 基金项目:国家自然科学基金项目（31660062）；内蒙古自治区高等学校科学研究项目（NJZZ17160） |
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| Regulation of VIPP1 dynamics and its effect on chloroplast envelope repair |
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XU Yilong,MA Lijuan,ZHANG Lingang,et al
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| Abstract: |
| 【Objective】This study investigated vesicle inducing protein in plastid 1 (VIPP1) dynamics and clarified the molecular mechanism of repairing chloroplast envelop.【Method】The intact chloroplasts were isolated from transgenic line VIPP1-GFP/Col of Arabidopsis.The detection of movement of VIPP1-GFP protein complex was carried out under fluorescence microscope with or without GTP and/or Ca2+.The dynamics mode of VPP1-GFP was analyzed and its mechanism was discussed.【Result】Chloroplasts began to swollen up and the gap between envelop and thylakoid enlarged under hypotonic stress conditions.VPP1-GFP protein complex started moving freely inside the chloroplast.CaCl2 could completely inhibit the movement of VPP1-GFP protein, which was caused by Ca2+ rather than Cl-.GTP effectively reversed the inhibitory effect of Ca2+ on VPP1-GFP movement and activated protein dynamics again.The integrity of chloroplast envelop was dependent on the dynamics of VIPP1 under hypotonic stress condition.【Conclusion】Both GTP and Ca2+ could regulate VIPP1 dynamics in Arabidopsis chloroplast.GTP promoted the movement of VIPP1 protein, while Ca2+ inhibited the process. |
| Key words: chloroplast envelop VIPP1 movement chloroplast repairment |
