| 摘要: |
| 【目的】研究临床分离的大肠杆菌多重耐药株（R）与大肠杆菌标准株ATCC 25922（S）蛋白质差异表达情况，为进一步研究大肠杆菌耐药机理奠定基础。【方法】采用串联质谱标签（tandem mass tag,TMT）法结合平行反应监测（parallel reaction monitoring,PRM）技术，对大肠杆菌R和S株的全菌体蛋白进行定量蛋白质组学研究，筛选大肠杆菌多重耐药株(S)与敏感株(R)之间的差异表达蛋白，并对其生物信息学进行分析。【结果】共筛选出300个差异表达蛋白（Fold change≥1.3，P<0.05），其中上调167个，下调133个，涉及的耐药相关通路主要包括细菌趋药性、ABC转运蛋白、β-内酰胺抗性等；PRM成功定量到13个差异蛋白，且PRM验证结果与TMT定量结果一致。【结论】筛选出多个与大肠杆菌耐药性相关的差异表达蛋白和通路。 |
| 关键词: 大肠杆菌 耐药性 蛋白质组学 |
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| 基金项目:国家自然科学基金项目（31772802）；安徽高校自然科学重点研究项目（KJ2017A132） |
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| Proteomics difference between Escherichia coli multidrug resistant and susceptible strains |
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ZHAO Xia,LI Rui,GAO Haixia,et al
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| Abstract: |
| 【Objective】The differential expression of proteins between clinically isolated multi-drug resistant Escherichia coli strain (R) and Escherichia coli ATCC 25922 (S) was studied to lay foundation for further study on drug resistance mechanism of Escherichia coli.【Method】Tandem mass tag (TMT) method combined with parallel reaction monitoring (PRM) technology was used to carry out quantitative proteomics on the whole bacterial protein of Escherichia coli R and S strains.Differentially expressed proteins between multi-drug resistant strains and sensitive strains were screened and bioinformatics analysis was conducted.【Result】A total of 300 differentially expressed proteins (Fold change≥1.3,P<0.05) were screened,of which 167 were up-regulated and 133 were down-regulated.Related pathways of drug resistance mainly included bacterial chemotaxis,ABC transporter,and β-lactam resistance.PRM successfully quantified 13 proteins and the validation results were consistent with TMT quantitative results.【Conclusion】Several differentially expressed proteins and pathways related to drug resistance of Escherichia coli were screened out. |
| Key words: Escherichia coli drug resistance proteomics |
