| 摘要: |
| 【目的】明确不同失水处理对采后烟叶蔗糖代谢的影响，为优质烤烟形成奠定基础。【方法】以烤烟品种“秦烟96”为材料，选取适熟中部烟叶（10～12叶位），设置正常处理（CK）、失水10%处理（T1）和失水20%处理（T2）3个失水梯度，在处理后不同时间测定烟叶蔗糖、葡萄糖和果糖含量，研究采后烟叶蔗糖磷酸合成酶（SPS）、蔗糖合成酶（SS）和转化酶（INV）活性及基因表达的动态变化。【结果】与正常处理（CK）相比，失水处理总体上增加了采后烟叶蔗糖、葡萄糖和果糖含量。失水10%处理烟叶的SPS活性随采后时间的延长呈逐渐下降趋势，且SPS基因表达量降低，而SS和INV活性随采后时间的延长呈上升趋势，SS和INV基因表达量增加；失水20%处理烟叶的SPS、SS和INV活性及基因表达量则与失水10%处理呈相反变化规律。【结论】失水处理促进了采后烟叶糖含量增加，且失水10%处理烟叶在采后以蔗糖分解代谢为主，而失水20%处理则抑制了蔗糖分解代谢。 |
| 关键词: 快速失水 烟叶调制 蔗糖代谢 蔗糖代谢酶 |
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| 基金项目:河南省高等学校青年骨干教师资助项目（2015GGJS-079） |
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| Effects of rapid water loss on sucrose metabolism in postharvest tobacco leaves |
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WU Feiyue,ZHOU Zifang,GAO Yabei,et al
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| Abstract: |
| 【Objective】This study identified the effects of different water loss treatments on sucrose metabolism in postharvest tobacco leaves to lay a foundation for formation of high quality flue cured tobacco.【Method】The flue cured tobacco variety “Qinyan 96” was selected and appropriate middle tobacco leaves (10-12 leaves) were taken for three water loss gradients of normal treatment (CK),water loss 10% (T1) and water loss 20% (T2).The contents of sucrose,glucose and fructose in tobacco leaves were determined and sucrose phosphate synthase (SPS),sucrose synthase (SS) and invertase (INV) activities and gene expression in postharvest tobacco leaves were studied.【Result】Compared with CK,water loss treatments generally increased contents of sucrose,glucose and fructose in postharvest tobacco leaves.The SPS activity of 10% dehydration treatment decreased gradually with the prolongation of post-harvest time,and the expression of SPS gene decreased.The activities of SS and INV increased with the prolongation of post-harvest time,and the expression levels of SS and INV genes increased.The SPS,SS and INV enzyme activities and gene expression levels of tobacco treated with 20% water loss were opposite to those treated with 10% water loss.【Conclusion】Dehydration treatment promoted the accumulation of post-harvest leaf sucrose content.The 10% dehydration treatment was mainly carbohydrate catabolism in postharvest leaves,while the 20% dehydration treatment inhibited sucrose decomposition metabolism. |
| Key words: rapid water loss tobacco leaf modulation sucrose metabolism sucrose metabolism enzymes |
