| 摘要: |
| 【目的】研究白菜叶片在不同贮藏温度下超氧化物歧化酶（SOD）、过氧化物酶（POD）活性及丙二醛（MDA）含量的变化特点。【方法】以白菜品种紫衣、百慕田和947-5s为供试材料，将白菜叶片在不同温度下贮藏不同时间（4 ℃下贮藏0，3，5，8和10 d－40－80 ℃下贮藏0，12，24，48和72 d），测定SOD、POD活性及MDA含量。【结果】不同品种叶片的SOD活性明显不同。4 ℃贮藏条件下，紫衣、百慕田和947 5s SOD活性表现相同的变化趋势，即冷藏0～5 d和8～10 d活性变化不大，冷藏5～8 d SOD活性明显增大，8 d时SOD活性较5 d时分别提高了62.28%，74.83%和61.96%；－40，－80 ℃两种冻藏温度下，SOD活性均表现为升高 降低 上升的变化趋势。不同品种叶片的POD活性明显不同。4 ℃贮藏条件下，紫衣、百慕田和947-5s POD活性都表现先下降再上升趋势，在冷藏第3天降到最低值，较0 d分别下降了42.64%，18.16%和11.4%，3～10 d POD活性逐渐上升，10 d时的POD活性分别为0 d的1.10，1.49和2.14倍；在两种冻藏温度下3个品种POD活性都呈现下降-上升-下降趋势，且品种间POD活性变化较大。3个白菜品种鲜叶片的MDA含量明显不同。4 ℃贮藏条件下，947 5s和百慕田MDA含量呈逐渐升高趋势，紫衣则为逐渐降低趋势。在－40，－80 ℃条件下，紫衣MDA含量都呈现下降-上升-下降-上升的波浪式趋势；－40 ℃条件下947-5s和百慕田MDA含量呈现上升-下降-上升相同趋势；－80 ℃条件下947 5s MDA含量呈现升高-降低-上升-降低趋势，而百慕田MDA含量无明显变化。【结论】3个白菜品种鲜叶片SOD、POD活性和MDA含量各不相同。3个白菜品种叶片只有SOD的活性在4 ℃冷藏0～5 d时有平稳期，而POD活性及MDA含量在冷、冻藏条件下都会随着时间延长而变化。 |
| 关键词: 白菜贮藏 超氧化物歧化酶 过氧化物酶 丙二醛 |
| DOI： |
| 分类号: |
| 基金项目:国家重点研发计划项目（2016YFD0101701)；陕西省蔬菜产业体系项目 |
|
| Effects of storage temperature on SOD and POD activities and MDA contents in Chinese cabbage leaves |
|
ZHANG Jie,SUN Yeshuo,XUE Yihua,et al
|
| Abstract: |
| 【Objective】The changes in activities of superoxide dismutase (SOD) and peroxidase (POD) and malondialdehyde (MDA) contents of Chinese cabbage leaves under different storage temperatures were studied.【Method】SOD and POD activities and MDA contents in leaves of Chinese Cabbage varieties (Ziyi,Baimutian and 947 5s) were detected and analyzed at different storage times and temperatures (4 ℃ for 0,3,5,8 and 10 d;-40 and -80 ℃ for 0,12,24,48 and 72 d).【Result】SOD activities in leaves of different varieties were significantly different.Under 4 ℃,SOD activities in all varieties showed the same trend with no significant change at 0-5 d and 8-10 d but significant increase at 5-8 d.SOD activities at 5-8 d were increased by 62.28%,74.83% and 61.96% for the three varieties compared with those at 5 d.Under -40 and -80 ℃,SOD activities showed the increase-decrease increase trend.POD activities were also different among varieties. Under 4 ℃,POD activities of all varieties showed decrease-increase trend.The lowest values at 3 d were 42.64%,18.16% and 11.4% lower compared with that at 0 d.POD activities gradually increased on 3-10 d.At 10 d,POD activities were 1.10,1.49 and 2.14 times of those at 0 d.Under -40 and -80 ℃,POD activities of all varieties showed a decrease-increase-decrease trend with significant differences among varieties.MDA contents in leaves were significantly different among varieties.Under 4 ℃,MDA contents in 947-5s and Baimutian showed a gradually upward trend,while that in Ziyi had a downward trend.Under -40 and -80 ℃,MDA content in Ziyi showed a decrease-increase-decrease-increase trend.Under -40 ℃,MDA contents in 947-5s and Baimutian showed increase-decrease-increase trend. Under -80 ℃,MDA content of 947-5s showed an increase-decrease-increase-decrease trend,while that of Baimutian had no change.【Conclusion】The activities of SOD and POD and contents of MDA were different in fresh leaves of the three Chinese cabbage varieties.Activities of SOD were not significantly changed for 0-5 d under 4 ℃,while POD activities and MDA content changed over time under cold and frozen conditions. |
| Key words: Chinese cabbage storage superoxide dismutase peroxidase malondialdehyde |
