| 摘要: |
| 【目的】优化聚酰胺树脂纯化茶枝柑叶总黄酮的工艺，为茶枝柑叶的开发利用提供参考。【方法】以总黄酮吸附量及解吸率为指标，通过静态吸附与解吸试验，确定适合分离纯化茶枝柑叶总黄酮的聚酰胺树脂粒径。通过动态吸附与解吸试验，采用单因素试验与响应面法优化，研究聚酰胺树脂分离纯化茶枝柑叶总黄酮的工艺参数，并对最优工艺条件进行验证。【结果】200~300目（48~75 μm）聚酰胺树脂较适用于纯化茶枝柑叶总黄酮。茶枝柑叶总黄酮最优纯化工艺为：上样液质量浓度（生药量）15 mg/mL，pH值为5，流速为1.75 BV/h，上样量为聚酰胺树脂质量的6倍（湿质量），用1.3 BV的75%（体积分数）乙醇洗脱。在该工艺条件下，所得纯化物中总黄酮得率为64.17%。【结论】200~300目（48~75 μm）聚酰胺树脂适用于茶枝柑叶总黄酮的分离纯化，且所得总黄酮纯度较高。 |
| 关键词: 茶枝柑 总黄酮 聚酰胺树脂 纯化工艺 |
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| 基金项目:广东省中医药建设专项资金面上项目（20171082）；广州中医药大学2017年校级大学生创新创业训练计划项目（201710572208） |
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| Purification technology of total flavonoids from Citrus reticulata ‘Chachi’ leaves |
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WANG Jinyu,ZHANG Qiuxia,CHEN Kang,et al
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| Abstract: |
| 【Objective】This study optimized the purification condition of total flavonoids from Citrus reticulata ‘Chachi’ leaves by polyamide resin.【Method】The adsorption rate and desorption rate of total flavonoids were used as indicators to determine the type of polyamide for static adsorption and desorption experiments.Through dynamic adsorption and desorption tests,single factor experiments and response surface methodology were used to optimize and determine the process parameters.【Result】The optimal conditions were:mesh of polyamide resin 48-75 μm,mass concentration of the sample solution (raw drug dosage) 15 mg/mL,pH 5,flow rate 1.75 BV/h,sample volume 6 times polyamide resin volume (wet weight),and elution by 75% ethanol for 1.3 BV.Under these conditions,the extract rate of total flavonoids was 64.17%.【Conclusion】The 48-75 μm polyamide resin was suitable for separation and purification of total flavonoids from leaves of Citrus reticulata ‘Chachi’. |
| Key words: Citrus reticulata ‘Chachi’ total flavonoids polyamide resin purification technology |
