| 摘要: |
| 【目的】研究紫花苜蓿(Medicago sative L.)细胞质雄性不育(CMS)的机理，为紫花苜蓿利用不育系的育种工作提供理论基础。【方法】本研究以紫花苜蓿细胞质雄性不育系MSGN1A及其保持系MSGN1B为材料，进行转录组测序（RNA-Seq）以及基因功能注释，筛选与不育系机理相关的差异表达基因及代谢通路。【结果】Illumina测序共得到紫花苜蓿95 679条功能基因，经过差异表达分析筛选出187个显著差异表达的基因(DEGs)，其中包括18个上调基因，169个下调基因。采用GO、KEGG、COG注释库分别对187个DEGs进行功能注释，其主要涉及催化活性、代谢活动、信号传导机制、合成、膜功能、碳水化合物运输和代谢、能量代谢等相关通路。【结论】筛选出与紫花苜蓿细胞质雄性不育性相关的代谢通路，初步探讨了紫花苜蓿细胞质雄性不育系的分子机理。 |
| 关键词: 紫花苜蓿 细胞质雄性不育 转录组测序 |
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| 基金项目:国家自然科学基金项目（31402125）；吉林省教育厅“十三五”科学技术项目（JJKH20180666KJ）；国家牧草产业技术体系项目（CARS35-02）；公益性行业（农业）科研专项“东北寒冷半湿润区苜蓿高效种植技术研究与示范”（201403048-0400） |
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| Identification of metabolic pathways related to cytoplasmic male sterility of Medicago sativa L. by transcriptome sequencing |
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CHEN Jingjing,WANG Yingzhe,GUO Qiang,et al
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| Abstract: |
| 【Objective】The cytoplasmic male sterility (CMS) mechanism was studied to enhance the use of sterile lines of alfalfa in breeding.【Method】RNA sequencing was performed to identify genes involving in CMS line MSGN1A and maintainer line MSGN1B.The genes function annotation analysis was conducted and the differences in gene expression and correlated metabolism pathways were investigated.【Result】A total of 95 679 unigenes were obtained by Illumina sequencing and 187 differentially expressed genes (DEGs) were identified with 18 up-regulated genes and 169 down-regulated genes. Based on GO,KEGG,and COG database,these DEGs were related to catalytic activity,metabolic process,signal transduction mechanisms,binding,membrance function,carbohydrate transport and metabolism,as well as energy metabolism.【Conclusion】The CMS related metabolism pathways were identified and the molecular mechanism of sterile lines of alfalfa was preliminarily revealed. |
| Key words: Medicago sativa L. cytoplasmic male sterility transcriptome sequencing |
