| 摘要: |
| 【目的】探究近年来乌蒙半细毛羊出现贫血、黄疸及血尿等症状的病因，为相关疾病防治提供参考。【方法】将出现患病羊的贵州省威宁县牛棚镇牧场作为试验牧场，贵州省赫章县凉水沟种羊场作为对照牧场，分别采集2个牧场的土壤、牧草样品，以及患病乌蒙半细毛羊（20只）与健康乌蒙半细毛羊（20只）的颈静脉血及肝脏，测定Cu、Mo、Mn、Co、Se、P、Fe和Zn含量，同时测定试验羊血液中SOD、CAT、GSH Px、CPK、GOT活性和CP、MDA、Hb含量及RBC、MCHC、MCV、PCV、MCH、WBC等指标；然后将健康乌蒙半细毛羊转移到试验牧场上放牧，10 d后进行钼酸铵补充试验，设0，100，200，300 mg/kg 4个处理，测定乌蒙半细毛羊血液和肝脏中铜含量的变化情况，分析钼元素的影响。【结果】试验牧场土壤中的铜含量极显著高于对照牧场（P<0.01），属于铜污染土壤；试验牧场牧草中的铜含量极显著高于对照牧场牧草（P<0.01），且试验牧场牧草中铜钼比高于动物饲粮中的铜钼比正常值；患病乌蒙半细毛羊血液和肝脏中的铜含量极显著高于健康羊（P<0.01），其他元素含量间无显著性差异；患病乌蒙半细毛羊血液中的SOD、CAT和GSH-Px活性极显著低于健康羊（P<0.01），CPK、GOT活性和CP、MDA含量极显著高于健康羊（P<0.01）；患病乌蒙半细毛羊血液Hb、RBC和MCHC极显著低于健康羊（P<0.01），MCV极显著高于健康羊（P<0.01），PCV、MCH和WBC与健康羊无显著性差异（P>0.05）。将健康乌蒙半细毛羊在试验牧场饲养后，出现了与患病乌蒙半细毛羊相同的症状，补充钼元素后，这些羊血液和肝脏中的铜含量明显降低，到试验结束时，体内铜含量恢复到正常范围，病症消失，且以每10 d补充300 mg/kg钼酸铵2次的效果最好。【结论】乌蒙半细毛羊的慢性铜中毒症状是采食含铜量过多的牧草造成的，在饲粮中补充300 mg/kg的钼酸铵，可以有效缓解相关症状。 |
| 关键词: 乌蒙半细毛羊 生理生化指标 矿物质元素 慢性铜中毒 |
| DOI： |
| 分类号: |
| 基金项目:国家“十三五”重点研发计划项目（2016YFC0502601）；西南科技大学博士基金项目（17zx7146） |
|
| Chronic copper poisoning of Wumeng semi-fine wool sheep |
|
WU Ting,HUO Bin,CHI Yongkuan,et al
|
| Abstract: |
| 【Objective】This study investigated the etiology of anemia,jaundice,and hematuria symptoms in Wumeng semi-fine wool sheep in recent years for preventing diseases.【Method】The Wumeng semi-fine wool sheep from the experimental pasture in Niupeng town of Weining,Guizhou were selected using those in the Liangshuigou sheep farm of Hezhang,Guizhou as the control.Soil,forage,and jugular vein blood and liver of 20 sick sheep and 20 healthy sheep were collected.Contents of Cu,Mo,Mn,Co,Se,P,Fe and Zn,activities of SOD,CAT,GSH-Px,CPK,GOT and CP,contents of MDA and Hb,and blood indexes of RBC,MCHC,MCV,PCV,MCH and WBC were determined and compared.Then,healthy sheep were moved to the experimental pasture for grazing.After 10 days,the ammonium molybdate supplement (0,100,200,and 300 mg/kg) tests were conducted and the changes of copper contents in blood and liver were analyzed to determine the effects of molybdenum.【Result】The soil in the experimental pasture was copper contaminated with content of copper significantly higher than that of the control pasture (P<0.01).The content of Cu in the forage of the experimental group was significantly higher than that in the control group (P<0.01),and the ratio of Cu to Mo in the forage of the experimental group was higher than that in animal diet.The concentrations of Cu in blood and liver of sick group were significantly higher than those of the health group,while there was no significant difference in contents of other elements between the two groups.SOD,CAT and GSH Px activities in the blood of sick sheep were significantly lower than those of the health group (P<0.01),while activities of CPK and GOT and contents of CP and MDA were significantly higher than those of the health group (P<0.01).Hb,RBC,and MCHC in blood of sick sheep were significantly lower than those of the health sheep (P<0.01),and MCV was significantly higher than health sheep (P<0.01).The contents of PCV,MCH and WBC showed no significant differences (P>0.05).When the healthy sheep were stocked in experimental pasture,the same symptoms as sick sheep appeared.After supplementing Mo with ammonium molybdate,the contents of Cu in the blood and liver reduced and the symptoms gradually disappeared.At the end of the experiment,the contents of Cu in sheep changed to normal level and the treatment with 300 mg/kg ammonium molybdate twice every 10 days was the best.【Conclusion】The symptoms of chronic copper poisoning in Wumeng semi-fine wool sheep were caused by long-term living on copper contaminated land.Supplementing 300 mg/kg ammonium molybdate to diet could effectively alleviate the symptoms. |
| Key words: Wumeng semi-fine wool sheep physiological and biochemical indexes mineral element chronic copper poisoning |
