| 摘要: |
| 【目的】探究加味四君子汤对脾虚犬相关指标及功能的影响，为临床脾虚犬的治疗提供参考。【方法】选择24只1岁龄的比格犬，随机平均分为4组，分别为对照组、脾虚组、自然恢复组、加味四君子汤恢复组。对照组犬饲喂基础饲粮；其余3组犬先饲喂基础饲粮并每天灌喂6 mL/kg番泻叶水煎液，上下午各1次，构建脾虚模型。模型构建成功后，将脾虚组犬解剖采样，加味四君子汤恢复组饲喂添加加味四君子汤的饲粮(每50 g添加1 mL)，自然恢复组饲喂基础饲粮。分别在第0，7，14天采血，测定血常规指标(白细胞(WBC)数、淋巴细胞(LYM)数、中性粒细胞(Gran)数、红细胞(RBC)数、血红蛋白(HGB)含量、红细胞压积(HCT)、血小板(PLT)数)，并分离血清测定生化指标(丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性及总蛋白(TP)、白蛋白(ALB)、血糖(GLU)、胆红素(TBIL)含量)以及D-木糖含量。第14天，全部犬解剖采集小肠和胰腺组织，观察小肠形态并测定胰腺消化酶活性。【结果】番泻叶水煎液灌喂第7天，试验犬出现脾虚证候群，表明脾虚模型构建成功。第0天时，各组犬各类指标均无显著差异。第7天时，与对照组相比，脾虚犬体质量极显著降低(P＜0.01)；血液中WBC、LYM数量，血清TP含量及胰腺组织中淀粉酶、脂肪酶、胃蛋白酶活性显著降低(P＜0.05)，RBC数量、HGB含量、HCT及血清ALB、GLU、D-木糖含量极显著降低(P＜0.01)，ACT活性显著升高(P＜0.05)、胆红素含量极显著升高(P＜0.01)。电镜观察发现，脾虚组犬小肠绒毛稀疏且部分断裂、缺损，细胞器结构损伤。第14天时，脾虚犬经加味四君子汤治疗后，各项指标均恢复到对照组水平。【结论】加味四君子汤能够使脾虚犬的生理生化指标及消化吸收功能恢复正常，对犬脾虚证有明显治疗效果。 |
| 关键词: 脾虚证；加味四君子汤；生理生化指标；消化吸收功能 犬 |
| DOI： |
| 分类号: |
| 基金项目:国家重点研发计划项目（2016YFD0501009） |
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| Effects of supplementary Sijunzi decoction on blood physiological and biochemical indexes and digestive and absorptive functions of splenic asthenia canines |
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HE Yanting,XIA Xiaodong,SHU Yingshuang,et al
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| Abstract: |
| 【Objective】The present study was conducted to investigate the effects of supplementary Sijunzi decoction on some indexes as well as digestive and absorptive functions of splenic asthenia canines,and to provide reference for clinial care of splenic asthenia canines.【Method】Twenty-four one-year-old beagles were selected and randomly divided into four groups with six per group.The four groups were control group,splenic asthenia group,natural recovery group and supplementary Sijunzi decoction recovery group,respectively.The canines in control group were fed on a basal diet,and those in the other three groups were fed on a basal diet with 6 mL/kg decoction per day at morning and afternoon to establish spleen deficiency model.After the model was developed,the canines in splenic asthenia group were dissected for collecting samples,those in natural recovery group were fed a basal diet,while those in supplementary Sijunzi decoction recovery group were fed on a basal diet with 1 mL per 50 g supplementary Sijunzi decoction.On days 0,7 and 14,blood samples were taken to measure blood routine indexes (white blood cell (WBC) count,lymphocyte (LYM) count,neutrophilicgranulocyte (Gran) count,red blood cell (RBC) count,hemoglobin (HGB) content,erythrocyte (HCT) and platelets (PLT) count),and sera were separated to measure biochemical indexes (activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST),and contents of total protein (TP),albumin (ALB),blood glucose (GLU) and total bilirubin (TBIL)) and D xylose content. On day 14,all canines were dissected for collecting small intestine and pancreas tissues to observe morphology and detect pancreatic digestive enzyme activities.【Result】On day 7,the spleen deficiency syndrome was observed in tested canines,indicating that the spleen deficiency model was successfully developed.On day 0,there was no significant difference in all indicators among groups.On day 7,body weight of splenic asthenia canines was extremely significantly decreased compared with the canines in control group (P＜0.01).WBC count,LYM count,serum TP content and activities of lipase,amylase and trypsin in pancrease were significantly decreased (P＜0.05),RBC count,HGB content,HCT and contents of ALB,GLU and D xylose in serum were extremely significantly decreased (P＜0.01),AST activities were significantly increased (P＜0.05),and TBIL content was extremely significantly increased (P＜0.01).Structural damage and cell necrosis of villus in small intestines were occurred in the splenic asthenia group by scanning electron microscope.On day 14,all indexes of the splenic asthenia canines recovered to the control group level after treatment by supplementary Sijunzi decoction.【Conclusion】Supplementary Sijunzi decoction can improve blood physiological and biochemical indexes as well as digestive and absorptive capacity,and have significant effects on treating splenic asthenia canines. |
| Key words: splenasthenic syndrome supplementary Sijunzi decoction physiological and biochemical indexes digestive and absorptive capacity canines |
