| 摘要: |
| 【目的】研究党参总皂苷纳米乳（total saponins of Codonopsis pilosula nanoemulsion，TSCP-NE）对小鼠免疫功能的影响，为TSCP-NE作为免疫增强剂用于保健品或药品提供理论依据。【方法】将小鼠分为空白对照组、模型对照组、空白纳米乳组、TSCP水溶液组、TSCP-NE高中低剂量组，空白对照组和模型对照组小鼠灌胃蒸馏水，空白纳米乳组小鼠灌胃空白纳米乳，TSCP水溶液组灌胃200 mg/kg TSCP，TSCP-NE高中低剂量组分别灌胃200，100，50 mg/kg TSCP-NE，连续灌胃14 d（1次/d），用药量0.01 mL/g。除空白对照组外，其余各组小鼠在给药第1 天皮下注射氢化可的松（HY），连续注射7 d，制备免疫抑制小鼠，检测分析免疫抑制小鼠细胞免疫（血清IL-2和IFN-γ含量、ConA诱导脾细胞增殖活性）、体液免疫（血清溶血素水平）和非特异性免疫（NK细胞杀伤活性、单核巨噬细胞吞噬活性）相关指标。【结果】TSCP-NE为透明淡黄色O/W纳米乳，平均粒径为60.67 nm，粒径多分散值为0.347，折光率、pH值、粘度、浊点分别是1.340 6，6.79，4.38×10-3（Pa·s），60.5 ℃。50~200 mg/kg TSCP-NE能显著提高免疫抑制小鼠血清IL-2和IFN-γ水平、脾T细胞增殖刺激指数、NK细胞杀伤率、血清溶血素半数溶血值（HC50）、单核巨噬细胞廓清指数和吞噬指数；TSCP-NE免疫增强作用具有量效依赖性，随着给药剂量增加，免疫增强作用明显增大，且其作用显著强于同剂量TSCP水溶液。【结论】TSCP-NE能增强TSCP对细胞免疫、体液免疫和非特异性免疫的调节功能，可以作为免疫增强剂用于保健品或药品。 |
| 关键词: 党参总皂苷 纳米乳 免疫增强 免疫抑制 |
| DOI： |
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| 基金项目:山西省“131”领军人才工程资助项目（XK-2015015）；运城学院院级科研项目（博士启动项目） |
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| Effect of total saponins of Codonopsis pilosula nanoemulsion on immunologic function of mice |
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CAO Fahao,WANG Yanping
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| Abstract: |
| 【Objective】This paper studied the effects of total saponins of Codonopsis pilosula nanoemulsion (TSCP-NE）on the immunologic function of mice to provide theoretical basis for using TSCP-NE as an immune enhancer in health products or medicines.【Method】Mice were divided into blank control group, model control group,blank NE group,TSCP aqueous solution group,as well as high-,medium- and low-dose TSCP-NE groups.All mice were given with drugs by intragastric administration.The mice in blank control group and model control group were given with distilled water.The mice in blank NE group were given with blank NE.The mice in TSCP aqueous solution group were given with TSCP aqueous solution with the dose of 200 mg/kg.The mice in high-,medium-and low-dose TSCP-NE groups were given with TSCP-NE with the dose of 200,100,and 50 mg/kg, respectively.Each mouse was administrated with drug by gavages for 14 consecutive days (1 times/d) and the given dose was 0.01 mL/g.Except the blank control group, mice in other groups were subcutaneously injected with HY on the first day of administration and treated for seven days to obtain immunosuppressive mice.The related indexes of cell immunity (contents of serum IL-2 and IFN-γ,and proliferation activity of ConA induced splenocyte),humoral immunity (serum hemolysin level) and non specific immunity (killing activity of NK cells and phagocytic activity of mononuclear macrophage) were detected and analyzed.【Result】TSCP-NE was a transparent yellowish O/W nanoemulsion with an average particle size of 60.67 nm and a polydispersity of 0.347. Its refractive index,pH value,viscosity,and cloud point were 1.340 6,6.79, 4.38×10-3 (Pa·s),and 60.5 ℃,respectively.Doses of 50-200 mg/kg TSCP NE significantly increased contents of serum IL-2 and IFN-γ,proliferation stimulation index of spleen T cells,half hemolysis value of serum hemolysin (HC50),killing rate of NK cells as well as clearance index and phagocytosis index of mononuclear macrophages.The immunoenhancement activities of TSCP-NE were dose-dependent,and the immune enhancement was enhanced significantly with the increase of dosage.The immunoenhancement activities of TSCP-NE were stronger than that of TSCP solution at same dose.【Conclusion】TSCP-NE enhanced the immunomodulatory effect of TSCP on cellular immunity,humoral immunity and non-specific immunity,and it can be used as an immunoenhancer for health products or medicines. |
| Key words: total saponins of Codonopsis pilosula nanoemulsion immunoenhancement immunosuppression |
