| 摘要: |
| 【目的】分析腹泻仔猪周围环境菌群变化规律，为仔猪腹泻的诊断和治疗提供支持。【方法】采集健康对照和腹泻仔猪粪便、口腔以及产床和母猪乳头等样品，采用ERIC-PCR技术分析其菌群结构特征，并对粪便样品中的细菌进行分离和鉴定。【结果】对照组4类样品细菌ERIC-PCR指纹图谱十分相似，腹泻组各类样品之间电泳条带差异较大，出现了异常亮度的电泳条带，与对照组优势条带位置不同，其中口腔和母猪乳头部位电泳条带数显著低于对照组（P＜0.05），菌群多样性指数降低，优势度指数升高。从粪便样品中分离出2株可疑菌株b1和b2，细菌染色和生化试验初步证明分离株b1和b2分别符合大肠杆菌和奇异变形杆菌的特征；利用ERIC-PCR技术分析分离株b1和b2的指纹图谱，发现分离优势细菌的ERIC-PCR指纹图谱包含于粪便样品的ERIC-PCR图谱中，证明样品的ERIC-PCR指纹图谱能够反映出优势菌群的特征；测序结果显示，分离株b1与大肠杆菌基因组同源性为99%，分离株b2与奇异变形杆菌基因组同源性为98%。【结论】腹泻仔猪周围环境菌群发生明显变化，ERIC-PCR技术可以快速、准确地监测和诊断菌群结构的变化，有助于细菌性仔猪腹泻的诊断和治疗。 |
| 关键词: 仔猪腹泻 肠道菌群 ERIC-PCR 指纹图谱 |
| DOI： |
| 分类号: |
| 基金项目:陕西省科学技术研究发展计划项目（2012K02-03）；陕西省重点农业科技推广及示范项目（ZDKJ-2014-33） |
|
| ERIC-PCR fingerprint analysis of intestinal flora of diarrhea piglets |
|
ZHANG Zhencang,YANG Ershuai,GUO Kangkang,et al
|
| Abstract: |
| 【Objective】This study analyzed the changes of flora in the surrounding environment of diarrhea piglets to provide support for the diagnosis and treatment of diarrhea in piglets.【Method Samples were collected from feces,mouth,obstetric table and sow nipple of health and diarrhea piglets.The bacterial community structure characteristics in the samples were analyzed by using ERIC-PCR technology,and the bacteria in fecal samples were isolated and identified.【Result】The ERIC-PCR fingerprints of the 4 samples in control group were very similar,but the electrophoresis bands were different in diarrhea groups.Abnormal brightness electrophoretic bands appeared in diarrhea groups and the locations of bands were different from the control group.Especially,the mean numbers of bands in mouth and nipple samples were significantly lower than those of control group (P<0.05).In the diarrhea group,bacteria diversity index decreased,while the dominance index increased.Two suspicious strains b1 and b2 were separated from fecal samples of diarrhea piglets.Bacterial staining and biochemical experiments showed that strains b1 and b2 were identical with characteristics of Escherichia coli and Proteus.ERIC-PCR fingerprinting analysis showed that the ERIC-PCR fingerprints of the dominant bacteria were included in the ERIC-PCR map of the fecal samples,indicating that the ERIC-PCR fingerprint of the samples could reflect the characteristics of the dominant flora.The sequencing results showed that DNA sequences of bands in isolated b1 and b2 were in 99% and 98% homology with Escherichia coli and Proteus,respectively.【Conclusion】The surrounding environmental flora of diarrhea piglets changed significantly.The ERIC-PCR technology can quickly and accurately monitor and diagnose the bacterial community structure characteristics,which is helpful for diagnosis and treatment of bacterial diarrhea in piglets. |
| Key words: piglet diarrhea intestinal flora ERIC-PCR fingerprints |
