| 摘要: |
| 【目的】对1株寄生于枣树的野生粗毛纤孔菌进行鉴定和系统发育分析，探讨其深层发酵液的抗氧化与抗菌活性，为进一步开发利用粗毛纤孔菌提供科学依据。【方法】通过形态学特征试验和ITS rDNA序列分析，对野生粗毛纤孔菌进行鉴定；基于ITS1-5.8S-ITS2序列的长度、碱基构成和变异程度,对粗毛纤孔菌遗传多样性进行分析；测定野生粗毛纤孔菌深层发酵液提取物对羟基和DPPH自由基的清除率，以及其对肺炎克雷伯菌、铜绿假单胞杆菌、大肠杆菌和金黄色葡萄球菌的抑菌圈直径，以此分析其抗氧化、抗菌活性；通过检测经一定温度和紫外照射处理的菌株发酵液提取物的抗氧化、抗菌活性，分析其抗氧化、抗菌活性的稳定性。【结果】根据菌株形态学特征试验和ITS rDNA序列分析，将野生菌株鉴定为粗毛纤孔菌(Inonotus hispidus)。不同地域的粗毛纤孔菌ITS1-5.8S-ITS2序列长度在614~718 bp，GC含量在44.4%~51.8%，序列总变异率达52.70%，表明其在自然界中具有丰富的遗传多样性。粗毛纤孔菌深层发酵液提取物具有一定的抗氧化和抗菌活性，且活性与发酵液提取物质量浓度呈正相关，当质量浓度为2 mg/mL时，其对羟基自由基的清除率达到65.96%，对DPPH自由基清除率达91.91%；当质量浓度为10 mg/mL时，其对铜绿假单胞杆菌和金黄色葡萄球菌表现出一定的抑制作用，但对肺炎克雷伯菌和大肠杆菌未表现出抑制作用；其抗氧化与抗菌活性具有较好的稳定性。【结论】获得了粗毛纤孔菌遗传多样性的基础信息，其深层发酵液具有一定的抗氧化与抗菌活性。 |
| 关键词: 粗毛纤孔菌 遗传多样性 深层发酵液 生物活性 |
| DOI： |
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| 基金项目:国家自然科学基金项目（81503187）；福建省中青年教师教育科研项目（JA15177）；福建农林大学现代种业工程研究院建设经费支持项目；福建省重大农技推广服务试点项目（KNJ-153011-4）；福建省食用菌产业技术重大研发平台项目（2014N2001） |
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| Identification and biological activity of a medicinal Inonotus |
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JIN Shanshan,LIU Xin,HOU Ruolin,et al
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| Abstract: |
| 【Objective】A wild Inonotus hispidus strain parasitized on Jujube tree was identified and analyzed phylogenetically and the antioxidant and antibacterial activities of its submerged fermentation broth were analyzed to provide basis for its further development and utilization.【Method】The wild I. hispidus was identified by morphological characteristics and ITS rDNA sequences.The length,base composition,variation degree of ITS1-5.8S-ITS2 sequences were analyzed and compared to investigate the genetic diversity.The antioxidant activity of the submerged fermentation broth was determined by measuring its scavenging ability to hydroxyl radical and DPPH radical.The antibacterial activities against Klebsiella pneumoniae,Pseudomonas aeruginosa,Escherichia coli and Staphylococcus aureus were determined by measuring the diameter of inhibition zone.【Result】The wild strain parasitized on jujube tree was identified as I. hispidus.The length variation range of I. hispidus ITS1-5.8S-ITS2 sequence in different areas was 614-718 bp,the GC content was 44.4%- 51.8% and the total variation rate was 52.70%,indicating that I. hispidus had abundant genetic diversity in natural world.The submerged fermentation broth of I. hispidus had antioxidant and antibacterial activities,and the antioxidant and antibacterial activities were positively correlated with concentration.When the concentration was 2 mg/mL,the hydroxyl radical scavenging rate reached 65.96% and DPPH radical scavenging rate reached 91.91%.When the concentration was 10 mg/mL,it showed inhibitory effect on P. aeruginosa and S. aureus,while it showed no inhibitory effect on K. pneumoniae and E. coli.The antioxidant and antibacterial activities had good stability.【Conclusion】The basic information of the genetic diversity of the parasitized species was obtained,and its deep fermentation liquid had good antioxidant and antibacterial activities. |
| Key words: Inonotus hispidus genetic diversity submerged fermentation broth biological activity |
