| 摘要: |
| 【目的】构建黄瓜分子遗传图谱，对控制黄瓜嫩果白色皮色的QTL进行初步定位。【方法】以嫩果皮色不同的3份黄瓜种质Q8、Q16、Q24为亲本材料，分别配制2个杂交组合Q16×Q8和Q16×Q24及6世代遗传群体。利用色差仪和目测法观测黄瓜嫩果皮色；利用2个组合亲本进行多态引物筛选，进而进行遗传图谱的构建和嫩果白色皮色的QTL定位。【结果】121对SSR引物在Q16×Q8亲本间表现多态，多态引物的比率为12.2%；126对SSR引物在Q16×Q24亲本间表现多态，多态引物的比率为12.7%。在Q16×Q8组合的遗传图谱中，包含了7个连锁群，74个SSR标记覆盖的基因组总长度为2 400.73 cM；在Q16×Q24组合的遗传图谱中，包含7个连锁群，76个SSR标记覆盖的基因组总长度为1 623.37 cM。根据构建的连锁图，对黄瓜嫩果白色皮色的QTL进行了初步定位研究，在Q16×Q8组合的7个连锁群上共定位了2个QTL位点，分别位于第1与第3染色体上，第1染色体上的位点可解释9.276%的表型变异，第3染色体上的位点可解释50.622%的表型变异；在Q16×Q24组合的7个连锁群上共定位了1个QTL位点，位于第3染色体上，可解释68.976%的表型变异。【结论】从Q16×Q8 和Q16×Q24 2个杂交组合中共检测到3个控制黄瓜嫩果皮色的QTL位点。 |
| 关键词: 黄瓜嫩果皮色 遗传图谱构建 QTL定位 |
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| 基金项目:国家自然科学基金项目( 31572143)；陕西省农业科技计划攻关项目(2016NY-074) |
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| Preliminary study on QTL mapping of immature fruit white color in cucumber (Cucumis sativus L.) |
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ZHANG Tingting,CHEN Haixu,SHEN Xiaoqing,et al
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| Abstract: |
| 【Objective】This paper determined constructed two genetic linkage map and the detected the QTL of inmature fruit white color in cucumber.【Method】In the present study,two crosses include six populations were constructed by three inbred parental lines (Q16×Q8 and Q16×Q24) to investigate the inheritance patterns of immature fruit white color in cucumber.Then on this basis,the SSR markers polymorphic screened,linkage map construction and QTL analyses were executed respectively.【Result】The result showed that the cross of Q16×Q8 produced 121 pairs polymorphism primers,the polymorphism rate was 12.2%.And the cross of Q16×Q24 produced 126 pairs polymorphism primers,the polymorphism rate was 12.7%.The genetic linkage map of the cross Q16×Q8 contained 7 linkage groups and 74 SSR markers which spanned 2 400.73 cM.The genetic linkage map of the cross Q16×Q24 contained 7 linkage groups and 76 SSR markers which spanned 1 623.37 cM.We detected the QTL of tender fruit color in cucumber which based on the genetic linkage map.Result showed that two QTLs which located in LG1 and LG3 linkage group in cross Q16×Q8.The phenotypic variation which can explain was 9.276% and 50.622% respectively.The cross Q16×Q24 had one locus related to the cucumber fruit color on the LG3.The phenotypic variation which can explain was 68.976%.【Conclusion】A total of 3 QTLs were detected for immature fruit color trait. |
| Key words: cucumber immature fruit color linkage map construction QTL mapping |
