| 摘要: |
| 【目的】研究双酶法提取无花果汁的最佳工艺参数，为制备无花果汁、酿造无花果酒奠定基础。【方法】以无花果为材料，以出汁率为考察指标，通过单因素试验和响应面试验，研究纤维素酶和果胶酶添加量、酶解温度、酶解时间4个因素及其交互作用对无花果岀汁率的影响，利用响应面试验结果建立回归方程，并对回归方程进行显著性和方差分析，得到无花果果汁酶解提取最佳工艺参数并进行试验验证。【结果】通过单因素试验得到的无花果果汁最佳酶解提取条件为：纤维素酶添加量1.5%（质量分数，下同），果胶酶添加量0.3%，酶解温度55 ℃，酶解时间90 min。根据单因素试验结果进行响应面试验分析得出，无花果果汁的最佳酶解提取工艺条件为：纤维素酶添加量1.56%，果胶酶添加量0.28%，酶解温度53 ℃，酶解时间90 min；在此条件下无花果的岀汁率为72.15%，与理论值(73.99%)基本吻合，且比未处理无花果出汁率提高了75.46%。纤维素酶添加量与酶解温度和酶解时间、果胶酶添加量与酶解温度和酶解时间、酶解温度与酶解时间的交互作用均可在较大程度上影响无花果的岀汁率。【结论】通过响应面试验得到了双酶法提取无花果汁的最佳工艺参数，该工艺可以大幅提高无花果出汁率。 |
| 关键词: 无花果 纤维素酶 果胶酶 岀汁率 |
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| 基金项目:苏州市农业科技创新项目（SNG201653）；江苏农业科学院基金项目（6111682） |
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| Optimization of extraction enzymolysis process of fig juice ofFicus carica L.by response surface method |
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SUN Xiaohua,MA Yanhong,CUI Jin,et al
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| Abstract: |
| 【Objective】The optimum technological parameters for the extraction of fig juice by double enzyme method were obtained to provide foundation for the preparation of fig juice and the brewing of fig wine.【Method】The effects of four factors including cellulase addition amount,pectinase addition amount,enzymolysis temperature,enzymolysis time and their interaction on fig juice yield were studied by single factor test and response surface method (RSM).Regression equations were established and analyzed by significance and variance.The optimum technological parameters of enzymolysis were then obtained and verified by experiments.【Result】The optimum conditions of single factor experiment were 1.5%(mass fraction,the same below) cellulase addition amount,0.3% pectinase addition amount,55 ℃ enzymolysis temperature and 90 min enzymolysis time.The optimum conditions of enzymolysis by the response surface method were cellulase addition amount 1.56%,pectinase addition amount 0.28%,enzymolysis temperature 53 ℃,and enzymolysis time 90 min.The juice yield under optimal conditions was 72.15%,which was basically consistent with the theoretical value(73.99%) and was increased by 75.46% compared to that of untreated figs.The interactions between cellulase addition amount and enzymolysis temperature,cellulase addition amount and enzymolysis time,pectinase addition amount and enzymolysis temperature,pectinase addition amount and enzymolysis time,and enzymolysis temperature and enzymolysis time also significantly affected the juice yield.【Conclusion】The optimum processing parameters for extracting fig juice by double enzymatic method were obtained by response surface method,which greatly increased the juice yield. |
| Key words: Ficus carica L. cellulase pectinase juice yield |
